Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb

Catalog
2066
Select Your Size Price
200 uL (0.5 mg/mL)(2066) $475.00
20 uL (0.5 mg/mL)(2066S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.
Product Details
More Information
Applications Flow Cytometry
Clone PI3KY458-1A11
Format Unconjugated
Cross Reactivity Predicted to work with mouse, rat, and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85
Isotype Rabbit IgG1k
Preparation Protein A+G
Validated Reactivity Human, Mouse
Clonality Monoclonal
Recommended Usage 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
References 1. Cantley, L.C. (2002) Science 296, 1655-7.

2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.

3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
Storage -20ºC
Images
Cell Culture Products
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11. Cat.#2066.
Cell Culture Products
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.
Cell Culture Products
Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.