Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb
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Product Overview
Description
Phosphoinositidine 3-kinase (PI3K) targets phosphoinositide lipids for phosphorylation at the D-3 position to serve as a second messenger molecule to activate signaling pathways in response to extracellular stimuli. Akt/protein kinase B (PKB) is a major downstream target of PI3K. The multiple forms of PI3K have different specificities and different affinities for various phosphatidylinositol forms. The PH domains of downstream proteins in these pathways bind to these secondary messengers, causing altered cellular distribution and subsequent kinase activation. The PI3K/Akt pathway is altered in a large proportion of human cancers, as this pathway regulates cellular survival, cell cycle progression, and cell growth.
Product Details
| Specification | Description |
|---|---|
| Applications | Flow Cytometry, WB |
| Clone | PI3KY458-1A11 |
| Format | Unconjugated |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat, and other homologues. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85 |
| Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | -20ºC |
| Pseudonyms | Phosphatidylinositol 3-kinase regulatory subunit alpha, PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit beta, PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit gamma, PIK3R3, p55PIK |
| Uniprot ID | Q92569 P27986 O00459 |
| References | Franke TF, Kaplan DR, and Cantley LC. (1997) Cell. 88: 435-437. Fresno JAV, Casado E, Cejas P, et al. (2004) Cancer Treatment Reviews. 30: 193-204. |
Images
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.
Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.
Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11. Cat.#2066.
Western blot analysis of NIH3T3 cell extract, untreated or treated with UV using 0.5 ug/mL Phospho-PI3Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11. Cat #2066.
