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Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb

Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (1A11) rabbit mAb

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Catalog

2066

Select Your Size	Price
200 uL (0.5 mg/mL)(2066)	$475.00
20 uL (0.5 mg/mL)(2066S)	$175.00

Size

$175.00

Qty

Need to make a custom order?

Note

20 μL is enough antibody for at least 20 Western blots.

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Product Overview

Description	Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

Product Details

More Information
Applications	Flow Cytometry
Clone	PI3KY458-1A11
Format	Unconjugated
Cross Reactivity	Predicted to work with mouse, rat, and other homologues.
Detection	Anti-Rabbit IgG
Formulation	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Validated Reactivity	Human, Mouse
Clonality	Monoclonal
Recommended Usage	1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
References	1. Cantley, L.C. (2002) Science 296, 1655-7. 2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41. 3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
Storage	-20ºC

Images

Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11. Cat.#2066.

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.

Peptide blocking flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) antibody PI3KY458-1A11 at 0.1 µg/mL. Cat #2066.

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