Phospho-PDK1 (Ser241) (F7) rabbit mAb

Catalog
2296
Select Your Size Price
200 uL (0.5 mg/mL)(2296) $475.00
20 uL (0.5 mg/mL)(2296S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description PDK1 is a Ser/Thr kinase that is ubiquitously expressed throughout human tissues. PDK1 phosphorylates protein kinase B (PKB or Akt) at both Thr308 and Ser473 in vivo. PDK1 is active and phosphorylated in basal conditions, where it exists predominantly in the cytosol with a small fraction at the plasma membrane. This membrane association is likely mediated by PDK1 binding to phospholipids through its PH domain, which has been found to have strong affinity to specific phospholipids. PDK1 is a master regulator of at least 23 related protein kinases, and more than 50% of all human cancers show significant overstimulation of the PDK1 pathway. Most small-molecule inhibitors of PDK1 target the ATP binding site.
Product Details
More Information
Applications Flow Cytometry
Clone PDK1S241-F7
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser241 of human phospho PDK1
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms 3-phosphoinositide-dependent protein kinase 1, PDPK1
Uniprot ID O15530
References Peifer C and Alessi DR. (2008) ChemMedChem. 3: 1810-1838.
Vanhaesebroeck B and Alessi DR. (2000) Biochemical Journal. 346: 561-576.
Images
Cell Culture Products
Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or treated with K252 (red) or with pervanadate (green) using Phospho-PDPK1 (Ser241) antibody PDPK1S241-F7 at 0.1 µg/mL. Cat. #2296.
Cell Culture Products
Peptide blocking flow cytometric analysis of 293T cells secondary antibody only negative control (light blue) or treated with K252 (red) or with pervanadate (green) or K252 and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or K252 and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-NDRG1 (Thr346) antibody PDPK1S241-F7 at 0.1 µg/mL. Cat. #2296.