Phospho-p53 (Ser15) (1C11) rabbit mAb
Catalog #: 1186
20 μL is enough antibody for at least 20 Western blots.
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p53 is one of the most highly connected nodes in the signal transduction network in the cell, playing a major role in basic cellular functions. p53 is the first tumor-suppressor gene to be identified, and this gene atypically functions in most human cancers. p53 inactivation in these cancers can prevent formation of homo-tetramers, increase p53 degradation, sequester p53 outside the nucleus, or prevent DNA binding and downstream gene activation. DNA damage is one of the most extensively studied p53 activation mechanism. p53 phosphorylation at the N-terminus decreases affinity for MDM2, preventing p53 degradation and leading to excess accumulation. Phosphorylation at the carboxy terminus of p53 enhances DNA binding.
|Applications||Flow Cytometry, WB|
|Validated Reactivity||Human, Mouse|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser15 of human phospho p53.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Cellular tumor antigen p53, Tumor suppressor p53, TP53|
|References||Vogelstein B, Lane D, and Levine AJ. (2000) Nature. 408: 307-310.|
Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 µg/mL phospho-p53 (Ser15) antibody P53S15-1C11. Cat. #1186.
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-p53 (Ser15) antibody P53S15-1C11 at 0.1 µg/mL. Cat. #1186.
Flow cytometric analysis of SK-N-MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using phospho-p53 (Ser15) antibody P53S15-1C11 0.1 µg/mL. Cat. #1186.
Phospho-p53 (Ser15) (1C11) rabbit mAb FITC conjugateCatalog #: 1188
Phospho-p53 (Ser15) (1C11) rabbit mAb PE conjugateCatalog #: 1187
Phospho-p53 (Ser15) (1C11) rabbit mAb APC conjugateCatalog #: 1189