Phospho-p53 (Ser15) (1C11) rabbit mAb

Catalog
1186
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200 uL (0.5 mg/mL)(1186) $475.00
20 uL (0.5 mg/mL)(1186S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description p53 is one of the most highly connected nodes in the signal transduction network in the cell, playing a major role in basic cellular functions. p53 is the first tumor-suppressor gene to be identified, and this gene atypically functions in most human cancers. p53 inactivation in these cancers can prevent formation of homo-tetramers, increase p53 degradation, sequester p53 outside the nucleus, or prevent DNA binding and downstream gene activation. DNA damage is one of the most extensively studied p53 activation mechanism. p53 phosphorylation at the N-terminus decreases affinity for MDM2, preventing p53 degradation and leading to excess accumulation. Phosphorylation at the carboxy terminus of p53 enhances DNA binding.
Product Details
More Information
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Applications Flow Cytometry, WB
Clone P53S15-1C11
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser15 of human phospho p53.
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Cellular tumor antigen p53, Tumor suppressor p53, TP53
Uniprot ID P04637
References Vogelstein B, Lane D, and Levine AJ. (2000) Nature. 408: 307-310.
Images
Cell Culture Products
Flow cytometric analysis of SK-N-MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using phospho-p53 (Ser15) antibody P53S15-1C11 0.1 µg/mL. Cat. #1186.
Cell Culture Products
Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 µg/mL phospho-p53 (Ser15) antibody P53S15-1C11. Cat. #1186.
Cell Culture Products
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-p53 (Ser15) antibody P53S15-1C11 at 0.1 µg/mL. Cat. #1186.