Phospho-p53 (Ser15) (1C11) rabbit mAb

Name: Phospho-p53 (Ser15) (1C11) rabbit mAb
SKU: 1186

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Catalog

1186

Download Datasheet

Select Your Size	Price
200 uL (0.5 mg/mL)(1186)	$475.00
20 uL (0.5 mg/mL)(1186S)	$175.00

Size

$175.00

Qty

Need to make a custom order?

Note

20 μL is enough antibody for at least 20 Western blots.

Customers Also Viewed

Product Overview

Description	p53 is one of the most highly connected nodes in the signal transduction network in the cell, playing a major role in basic cellular functions. p53 is the first tumor-suppressor gene to be identified, and this gene atypically functions in most human cancers. p53 inactivation in these cancers can prevent formation of homo-tetramers, increase p53 degradation, sequester p53 outside the nucleus, or prevent DNA binding and downstream gene activation. DNA damage is one of the most extensively studied p53 activation mechanism. p53 phosphorylation at the N-terminus decreases affinity for MDM2, preventing p53 degradation and leading to excess accumulation. Phosphorylation at the carboxy terminus of p53 enhances DNA binding.

Product Details

More Information
Please Note	We are currently experiencing technical difficulties with our website that we hope to resolve soon. For order inquiries, please e-mail sales@abwizbio.com. International shipping is available.
Applications	Flow Cytometry, WB
Clone	P53S15-1C11
Format	Unconjugated
Validated Reactivity	Human, Mouse
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Clonality	Monoclonal
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser15 of human phospho p53.
Formulation	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Recommended Usage	1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage	-20ºC
Pseudonyms	Cellular tumor antigen p53, Tumor suppressor p53, TP53
Uniprot ID	P04637
References	Vogelstein B, Lane D, and Levine AJ. (2000) Nature. 408: 307-310.

Images

Flow cytometric analysis of SK-N-MC cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using phospho-p53 (Ser15) antibody P53S15-1C11 0.1 µg/mL. Cat. #1186.

Western blot analysis of L929 cell extract untreated or treated with etoposide using 0.05 µg/mL phospho-p53 (Ser15) antibody P53S15-1C11. Cat. #1186.

Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with staurosporine (green) using Phospho-p53 (Ser15) antibody P53S15-1C11 at 0.1 µg/mL. Cat. #1186.

Associated Products