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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (A11) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. The p44/42 MAPK (Erk1/2) are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. Phospho ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upstream MEK1 and MEK2 activate p44 and p42 (phospho ERK1/2) through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. p44/42 (ERK1/2) are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2.|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1- 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Warnecke N, et al. (2012) EMBO Rep. 13: 386-91.
2. Mebratu Y, et al. (2009) Cell Cycle 8:1168-75.
3. Ebisuya M, et al. (2005) J Cell Sci. 118: 2997-3002.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with U0126 (red) or treated with PMA (green) using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #1111.
Western blot analysis of Ramos cell extract untreated or treated with U0126 followed by no treatment or treatment with PMA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #1111.
Western blot analysis of 293T cell extract treated with U0126 or TPA using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 at 0.1 µg/mL. Cat. #1111.
ERK12T202Y204-A11 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of L929 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 (green) Cat. #1111.
Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with U0126 (red) or with PMA (green) using Phospho-Erk1/2 (T202/Y204) antibody ERK12T202Y204-A11 (Abwiz Cat. #1111) or Company C antibody at 0.43 µg/mL (manufacturer's recommended concentration).
Western blot analysis of Ramos cells treated with U0126 or treated with TPA using 0.05 ug/mL of Phospho ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11 Cat. #1111 or Company C antibody at recommended concentration of 0.34 ug/mL.
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