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Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb

Name: Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb
SKU: 2241

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2241

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Select Your Size	Price
200 uL (0.5 mg/mL)(2241)	$475.00
20 uL (0.5 mg/mL)(2241S)	$175.00

Size

$175.00

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Note

20 μL is enough antibody for at least 20 Western blots.

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Product Overview

Description	MKK3 and MKK6 are closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). Phospho MKK3 and phospho MKK6 both phosphorylate and activate p38. p38 phosphorylation dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Phospho MKK3 and phospho MKK6 activation occurs through phosphorylation at S189 and T222 on MKK3 (2) and S207 and T211 on MKK6 (4,5).

Product Details

More Information
Applications	Flow Cytometry, WB
Clone	MKK3S189MKK6S207-D3
Format	Unconjugated
Validated Reactivity	Human, Mouse
Cross Reactivity	Predicted to work with mouse, rat and other homologues.
Detection	Anti-Rabbit IgG
Clonality	Monoclonal
Immunogen	A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6.
Formulation	1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype	Rabbit IgG1k
Preparation	Protein A+G
Recommended Usage	1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage	-20ºC
Pseudonyms	Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK2, MAP2K3, MEK3, PRKMK3, SKK2
Uniprot ID	P46734
References	1. Derijard, B. et al. (1995) Science 267, 682-685. 2. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6. 3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384. 4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255. 5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

Images

Flow cytometric analysis of HEK293T cells secondary antibody only negative control (blue) or treated with K252a (red) or treated with UV+TPA (green) using 0.5 µg/mL of Phospho-MKK3(Ser189)/MKK6(Ser207) antibody MKK3S189MKK6S207-D3 Cat. #2241.

Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or treated with K252a (red) or UV/TPA-treated (green) or K252a and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or K252a and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 0.1µg/mL. Cat. # 2241.

MKK3S189MKK6S207-D3 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of MKK3(S189)/MKK6(S207) antibody MKK3S189MKK6S207-D3 (green) Cat. #2241.

Western blot analysis of COS7 cell extract untreated or treated with UV using 0.05 µg/mL Phospho-MKK3 (Ser189)MKK6(S207) antibody MKK3S189MKK6S207-D3. Cat. #2241.

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