Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D3) rabbit mAb
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2241
MKK3 and MKK6 are closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). Phospho MKK3 and phospho MKK6 both phosphorylate and activate p38. p38 phosphorylation dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Phospho MKK3 and phospho MKK6 activation occurs through phosphorylation at S189 and T222 on MKK3 (2) and S207 and T211 on MKK6 (4,5).
| Applications | Flow Cytometry, WB |
|---|---|
| Clone | MKK3S189MKK6S207-D3 |
| Format | Unconjugated |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser189 of human phospho MKK3 and Ser207 of human phospho MKK6. |
| Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | -20ºC |
| Pseudonyms | Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, Stress-activated protein kinase kinase 2, SAPK kinase 2, SAPKK2, MAP2K3, MEK3, PRKMK3, SKK2 |
| Uniprot ID | P46734 |
| References | 1. Derijard, B. et al. (1995) Science 267, 682-685. 2. Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6. 3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384. 4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255. 5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891. |
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