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Phospho-MEK1 (Ser298) (H8) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||MEK1 (also known as MAP2K1 or mitogen activated protein kinase kinase 1) and MEK2 are dual-specificity protein kinases that control cell differentiation and growth. phospho-MEK1/2 is activated by membrane depolarization, calcium influx, and a variety of growth factors and cytokines (1-3). phospho-MEK1/2 activates p44 and p42 MAP kinase by phosphorylating both Thr and Tyr residues within the activation loop. MEK1/2 activation occurs by Ser217 and Ser221 phosphorylation in the activation loop subdomain VIII by Raf-like molecules. PAK1 phosphorylates MEK1 at Ser298, promoting MEK1 binding to Raf, and promoting further phosphorylation of MEK1 by Raf (4).|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser298 of human phospho MEK1.|
|Validated Reactivity||Human, Rat|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Crews, C.M. et al. (1992) Science. 258:478-480.
2. Alessi, D.R. et al. (1994) EMBO J. 13:1610-1619.
3. Rosen, L.B. et al. (1994) Neuron. 12:1207-1221.
4. Coles, L.C. and Shaw, P.E. (2002) Oncogene. 21:2236-2244.
Flow cytometric analysis of Hela cells secondary antibody only negative control (blue) or treated with imatinib (red) or treated with pervanadate (green) using Phospho-MEK1 (S298) antibody MEK1S298-H8 0.5 µg/mL. Cat. #2266.
Western blot analysis of C6 cell extract untreated or treated with TPA using 0.01 µg/mL Phospho-MEK1 (S298) antibody MEK1S298-H8. Cat. #2266.
Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with MG132 (green) or treated and blocked with phospho peptide (orange) or treated and blocked with non-phospho peptide (purple) using Phospho-MEK1 (S298) antibody MEK1S298-H8 at 0.1 µg/mL. Cat. #2266.
Western blot analysis of C6 cell extract untreated or treated with TPA using 5 ng/mL Phospho-MEK1 (Ser298) antibody MEK1S98-H8. Cat.#2266 or Company C antibody at 104 ng/mL (manufacturer’s recommended concentration) developed using the same exposure.
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