Phospho-Chk2 (Thr68) (D12) rabbit mAb

Catalog #: 2116
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200 uL (0.5 mg/mL)(2116) $475.00
20 uL (0.5 mg/mL)(2116S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
Checkpoint kinase 2 (Chk2) plays a major role in the checkpoint response to DNA damage. Chk2 is initially inactive in its monomeric, unphosphorylated form. Phosphorylation at Thr68 induces homodimerization, initiating autophosphorylation within the kinase loop at Ser516 and phorphorylation events within the auto-inhibitory loop at Thr383 and Thr387. After these phosphorylations, active dimers and monomers can then phosphorylate substrates such as Cdc25C and BRCA1. In humans, Chk2 genetic deletion and missense variants have been found to be associated with increased risk of breast and colon cancer. Constitutively phosphorylated Chk2 at Thr68 has been found in many human cancer cell lines, especially ones with mutations in p53.

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone Chk2T68-D12
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat, and other homologues
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr68 of human phospho Chk2
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Serine/threonine-protein kinase Chk2, Cds1 homolog, Hucds2, CHEK2, RAD53
Uniprot ID O96017
References Ahn J, Urist M, and Prives C. (2004) DNA Repair. 3: 1039-1047.

Images

Cell Culture Products
Flow cytometric analysis of Hela cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using Phospho-CHK2 (Thr68) (D12) Rabbit mAb, at 0.01 ug/mL, CHK2T68-D12 #2116, or concentration-matched Rabbit (G9) mAb IgG Isotype Control #2141 for cells treated with lambda phosphatase (black) or treated with UV+TPA (blue).
Cell Culture Products
Peptide blocking flow cytometric analysis of HEK293T cells secondary antibody only negative control (light blue) or untreated (red) or UV/TPA-treated (green) or untreated and blocked with phospho-peptide (black) or UV/TPA and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or UV/TPA and blocked with non-phospho peptide (purple) using Phospho-Chk2 (T68) antibody Chk2T68-D12 0.1µg/mL. Cat. #2116.
Cell Culture Products
Chk2T68-D12 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of 3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-Chk2 (T68) antibody Chk2T68-D12 (green) Cat. #2116.
Cell Culture Products
Western blot analysis of HEK293 cell extract untreated or treated with UV using Phospho Chk2(T68) antibody Chk2T68-D12. Cat. #2116.
Cell Culture Products
Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 µg/mL Phospho-Chk2 (T68) antibody Chk2T68-D12 (Abwiz Cat. #2116) or Company C antibody at 2.3 µg/mL (manufacturer's recommended concentration).

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