Phospho-c-Cbl (Tyr700) (E1) rabbit mAb

Catalog
2321
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200 uL (0.5 mg/mL)(2321) $475.00
20 uL (0.5 mg/mL)(2321S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description The c-Cbl (Casitas B-lineage Lymphoma) proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that contains multiple functional domains, including an amino-terminal tyrosine kinase-binding (TKB) domain, a RING finger motif, and a proline-rich region. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) and on other nonreceptor tyrosine kinases, while the RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (1). The proline-rich region contains 14-3-3 protein-binding and SH3 domain-binding motifs. c-Cbl is phosphorylated at Y700, Y731, and Y774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for immunoglobulins, antigens, hormones, growth factors, and cytokines. Phosphorylated Y774 interacts with the SH2 domain of Crk (1,2). The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells (3,4). Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5).
Product Details
More Information
Applications Flow Cytometry
Clone CblY700-E1
Format Unconjugated
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr700 of human phospho c-Cbl
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase, Signal transduction protein CBL, CBL2, RNF55
Uniprot ID P22681
References 1. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2: 294-307.
2. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273: 8323-8331.
3. Blake, T.J. et al. (1991) Oncogene. 6: 653-657.
4. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76: 473-482.
5. Kamei, T. et al. (2000) Int. J. Oncol. 17: 335-339.
Images
Cell Culture Products
Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control Cat. #2141 or imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 µg/mL. Cat. #2321.
Cell Culture Products
Peptide blocking flow cytometric analysis of C6 cells, secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 µg/mL. Cat. #2321.
Cell Culture Products
Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or treated with imatinib (grey) or with pervanadate (orange) using 0.1 µg/mL isotype control Cat. #2141, or imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.1 µg/mL. Cat. #2321.
Cell Culture Products
Flow cytometric analysis of 3T3 cells, secondary antibody only negative control (blue) or treated with imatinib (red) or pervanadate (green) using Phospho-c-Cbl (Tyr700) antibody CblY700-E1 at 0.01 µg/mL. Cat. #2321.