Phospho-BLNK (Tyr84) (H4) rabbit mAb
Catalog #: 2291
20 μL is enough antibody for at least 20 Western blots.
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BLNK protein, known as SLP-65 play an important role as adaptor protein in B-lineage cells. BLNK associates with proteins in the cytoplasmic side of plasma membrane through its N-terminal leucine zipper motif. Upon BLNK activation on its tyrosine, BLNK binds to Btk, Vav, Brb2, Syk, and HPK1. Through this associations, BLNK mediates Ca2+ mobilization, for ERK1/2, JNK and p38 MAP kinase activation. After phosphorylation, BLNK binds Btk and PLCγ2 through their SH2 domains and mediates PLCγ2 activation by Btk. BLNK also binds other signaling molecules such as Vav, Grb2, Syk, and HPK1. BLNK plays aa important role in BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr84 of human phospho BLNK|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||B-cell linker protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP65, B-cell adapter containing a SH2 domain protein, BASH|
|References||Fu, C., et al. (1998) Immunity 9: 93–103.
Goitsuka, R., et al. (1998) J. Immunol. 161: 5804–5808.
Tsuji, S., et al. (2001) J. Exp. Med. 194: 529–539.
Peptide blocking flow cytometric analysis of Daudi cells secondary antibody only negative control (light blue) or untreated (red) or with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 µg/mL. Cat. #2291.
Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-BLNK (Tyr84) antibody BLNKY84-H4 at 0.01 µg/mL. Cat. #2291.
Flow cytometric analysis of Daudi cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNa + IL-4 + pervanadate (green) using 10 ng/mL of Phospho-BLNK (Tyr84) antibody BLNKY84-H4 (Abwiz Cat. #2291) or Company B antibody at 2.5 ug/mL (manufacturer's recommended concentration).
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