Phospho-BAD (Ser112) (B9) rabbit mAb
Catalog #: 2246
20 μL is enough antibody for at least 20 Western blots.
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Human BCL-2-assocaited death promoter (BAD) regulates apoptosis by binding to anti-apoptotic members of BCL family including BCL-2, BCL-xL, and BCL-w. The BAD protein signal transduction activity is regulated by multiple kinases and phosphatases. Non-phosphorylated BAD protein selectively dimerizes with Bcl-xL and Bcl-2 displacing Bax, which is then free to initiate mitochondrial membrane permeability, initiating apoptosis. When phosphorylated, BAD is unable to heterodimerize with Bcl-2 or Bcl-xL and is sequestered into the cytosolic compartment by binding to 14-3-3 protein. BAD protein has been shown to phosphorylated at multiple sites. The phosphorylation of 3 serine residues (Ser112, Ser136 and Ser155) effects the activity of the BAD. Multiple kinases phosphorylate BAD at these serine site. Ribosomal protein S6 kinase alpha-1 (RPS6KA1/RSK) and cAMP-dependent protein kinase phosphorylated BAD at Ser112 while protein kinase B (PKB/Akt) phosphorylates BAD at Ser136 residue. Phosphorylation of BAD at Ser-155 is preferentially carried out by Protein Kinase A. Other BAD phosphorylation sites may regulation cellular localization of BAD. Phosphorylation of both Ser75 and Ser99 increases sequestration of BAD by 14-3-3 protein which leads to translocation of BAD from the mitochondria to the cytosol. This prevents BAD from binding to anti-apoptotic proteins. Phosphorylation at Ser99 also prevents BAD from binding to the hydrophobic groove of Bcl-2 and Bcl-XL.
|Validated Reactivity||Human, Mouse, Rat|
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Ser112 of human phospho BAD|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, BBC6, BCL2L8|
|References||Czabotar PE., et al., (2014) Nat Rev Mol Cell Biol 15:49-63.
Polzien L., et al., (2009) J Biol Chem 284:28004-28020.
Masters SC., et al., (2001) Mol Pharmacol 60: 1325-1331.
Datta SR., et al., (1997) Cell 91:231-241.
Harada H., et al., (1999) Mol Cell 3:413-422.
Chiang CW et al., (2001) Blood 97:1289-1297.
Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using Phospho-BAD (Ser112) antibody BADS112-B9 at 0.1 µg/mL. Cat. #2246.
BADS112-B9 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or of Phospho-BAD (Ser112) antibody BADS112-B9 (green) Cat. #2246.
Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using Phospho-BAD (Ser112) antibody BADS112-B9 at 0.01µg/mL. Cat #2246.
Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using Phospho-BAD (S112) antibody BADS112-B9 (Abwiz Cat. #2246) or Company C antibody at 0.08 µg/mL (manufacturer's recommended concentration).
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