Phospho-ATF2 (Thr71) (G3) rabbit mAb

Catalog #: 2056
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200 uL (0.5 mg/mL)(2056) $475.00
20 uL (0.5 mg/mL)(2056S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description
Activating transcription factor 2 (ATF2), known as cyclic AMP (cAMP) response element (CRE) binding protein 2 (CREB2) and CRE-BP1 is a member of the activating protein-1 (AP1) transcription factor family. Through homo-dimerization or hetero-dimerization with other AP1 family members, such as the CREB, Fos, Maf, or Jun family transcription factor, it regulates the expression of many genes. ATF2 is an important mediator of mammalian cell responses to various stimuli, including stress. As a transcription factor, ATF2 acts as transducing extracellular signals to the nucleus to facilitate transcriptional responses to stimuli. ATF2 is stimulated by growth factors, ultraviolet (UV) radiation, and cytokines. Stress-activated protein kinases (SAPKs)(e.g. p38 MAP Kinase) induce transcriptional activation of ATF2. ATF2 is phosphorylated at amino acids threonine-69 and threonine-71 (T69, T71). Phosphorylation at these threonine residues is essential for maximal transcriptional activity downstream of insulin/epidermal growth factor stimulation and is dependent on cooperation between c-Jun N-terminal kinase (JNK) and signaling cascades downstream of Ras. Upon T69/T71 phosphorylation, ATF2 interacts with other AP1 proteins and translocate to the nucleus to regulate the expression of hundreds of genes.

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone ATF2T71-G3
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human phospho ATF2
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Cyclic AMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, HB16, CREB2, CREBP1
Uniprot ID P15336
References Shaulian E, and Karin M., (2002) Nat Cell Biol. 4:131–136.
Shimizu M, et al., (1998) Exp Cell Res. 239:93–103.
Lau E, and Ronai ZA. (2012) J Cell Sci. 125:2815–2824.
Bailey J, and Europe-Finner GN. (2005), J Mol Endocrinol. 34:19–35.
Raingeaud J, et al., (1995) J Biol Chem. 270:7420–7426.
Ouwens DM, et al., (2002) EMBO J. 21:3782–3793.
Livingstone C, et al., (1995) EMBO J. 1995,14:1785–97.
Yamasaki T, et al., (2009). J Biol Chem 284:8567–8581.
Lau E, et al., (2012) Cell 48:543–555.
Bhoumik A, et al., (2005) Mol Cell 18:577–587.

Images

Cell Culture Products
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat #2056.
Cell Culture Products
Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 µg/mL Phospho-ATF-2 (Thr71) antibody ATF2T71-G3. Cat. #2056.
Cell Culture Products
Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat. #2056.
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat #2056.
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (T71) antibody ATF2T71-G3 (Abwiz Cat. #2056) or Company C antibody at 0.07 µg/mL (manufacturer's recommended concentration).

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