Phospho-ATF2 (Thr71) (G3) rabbit mAb

Catalog
2056
Select Your Size Price
200 uL (0.5 mg/mL)(2056) $475.00
20 uL (0.5 mg/mL)(2056S) $175.00
$175.00
-
+

Need to make a custom order?

Contact us

Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description Activating transcription factor 2, also known as phospho ATF2, is a protein that, in humans, is encoded by the ATF2 gene.Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2/JUN complex. Cellular stress phosphorylates Thr69 and Thr71 and activates phospho ATF-2.
Product Details
More Information
Applications Flow Cytometry, WB
Clone ATF2T71-G3
Format Unconjugated
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr71 of human phospho ATF2
Isotype Rabbit IgG1k
Preparation Protein A+G
Validated Reactivity Human, Mouse
Clonality Monoclonal
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
References 1. Kawasaki H, et al. (2000) Nature 405:195-200.
2. Bhoumik A, Tet al. Ronai Z. (2005) Mol. Cell 18:577-87.
3. Bhoumik A, et al. (2008) J. Biol. Chem. 283:17605-14.
4. Lau E, et al., (2012) Cell 148: 543-55.
Storage -20ºC
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat #2056.
Cell Culture Products
Western blot analysis of 293T cell extract untreated or treated with UV using 0.1 µg/mL Phospho-ATF-2 (Thr71) antibody ATF2T71-G3. Cat. #2056.
Cell Culture Products
Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat. #2056.
Cell Culture Products
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or untreated (red) or treated with UV and PMA (green) using Phospho-ATF-2 (Thr71) antibody ATF2T71-G3 at 0.1µg/mL. Cat #2056.
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using Phospho-ATF-2 (T71) antibody ATF2T71-G3 (Abwiz Cat. #2056) or Company C antibody at 0.07 µg/mL (manufacturer's recommended concentration).