SEK1, in cooperation with MKK7, activates JNK by tyrosine phosphorylation in response to cellular stress signals, including osmotic stress, DNA damage, cytokines, and heat shock (1). SEK1 itself is activated by serine phosphorylation by MAP3Ks, including MEKK1, MLKs, ASK1, and TAK1 (2). In response to TNFα stimulation in melanoma cells, filamin interacts with SEK1 as a scaffold protein (3). SEK1 plays an important role in T cell signaling pathways. Both SEK1-dependent and independent pathways operate for SAPK activation in response to different cellular stresses and stimuli (4). SEK1 also plays an important role in embryonic development. SEK1 may activate c-Jun and c-Fos during mammalian embryonic liver development.
Predicted to work with mouse, rat and other homologues.
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser257 of human phospho SEK1/MKK4
Formulation
1X PBS, 0.09% NaN3, 0.2% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
1. Tibbles LA, et al., (1996) The EMBO Journal. 15:7026-7035.
2. Nishina H, et al., (1997) Nature. 385:350-353.
3. Hu L, et a., (2019) The JNK Pathway in Drug Resistance. Targeting Cell Survival Pathways to Enhance Response to Chemotherapy. 87-100.
4. Nishina H, et al. (1999) Development. 126:505-516.
