Phospho-SAPK/JNK (Thr183/Tyr185) (A11) rabbit mAb FITC conjugate
Customers Also Viewed
-
SARS-CoV2 LxC1-G10 human neutralizing mA...Catalog #: 2551 -
Human ACE2/ACEH Protein. Fc TagCatalog #: 2566 -
Phospho-S6-Ribosomal Protein (Ser240/244...Catalog #: 2374 -
Phospho-p38 MAPK (Thr180/Tyr182) (E3) ra...Catalog #: 1156
Product Overview
Description
The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFα or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.
Product Details
| Specification | Description |
|---|---|
| Applications | Flow Cytometry |
| Clone | SAPKT183Y185-A11 |
| Format | FITC |
| Validated Reactivity | Human |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | 2-8ºC |
| Pseudonyms | Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8 |
| Uniprot ID | P45983 |
| References | Verheij M, Bose R, Lin XH, et al. (1996) Nature. 380: 75-79. Verheij M, Ruiter GA, Zerp SF, et al. (1998) Radiotherapy and Oncology. 47: 225-232. Moriguchi T, Toyoshima F, Masuyama N, et al. (1997) The EMBO J 16: 7045-7053. |
Images
Flow cytometric analysis of 293T cells untreated and unstained as negative control (blue) or untreated (red) or with UV+TPA (green) and stained using Phospho-SAPK/JNK (Thr183/Tyr185) antibody SAPKT183Y185-A11 FITC conjugate. Cat. #2358.
Associated Products
-
Phospho-c-Cbl (Tyr700) (E1) rabbit mAb APC conjugateCatalog #: 2324 -
Phospho-c-Cbl (Tyr774) (R3B8) rabbit mAb FITC conjugateCatalog #: 2318 -
Phospho-PKC (pan) (gamma Thr514) (PF4) rabbit mAb APC conjugateCatalog #: 2454 -
Phospho-MAPKAPK2 (Thr334) (H2) rabbit mAb PE conjugateCatalog #: 2177