Phospho-SAPK/JNK (Thr183/Tyr185) (A11) rabbit mAb APC conjugate
The SAPK/JNK pathway initiates apoptosis upon exposure to radiation, UV exposure, heat shock, oxidative stress, and other stressors. Upon exposure to environmental stress, the SAPK/JNK signaling pathway sequentially activates the proteins MEKK1, SEK1, SAPK, and c-Jun. Upstream activators of the SAPK/JNK cascade include ceramide, small GTP-binding proteins such as Rac1 and Cdc42Hs, Ask1, and caspases. MKK7 is also a major and direct SAPK/JNK activator in the TNFα or environmental stress signaling pathways, where its kinase activity directly phosphorylates SAPK/JNK. This relationship between MKK7 and SAPK appears to be evolutionarily conserved, as it is preserved in their Drosophila homologues, Hep and DJNK, respectively.
|Cross Reactivity||Predicted to work with mouse, rat and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Thr183/Tyr185 of human phospho SAPK/JNK|
|Formulation||1X PBS, 0.09% NaN3, 0.2% BSA|
|Recommended Usage||For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Mitogen-activated protein kinase 8, MAPK8, Stress-activated protein kinase 1c, SAPK1c, c-Jun N-terminal kinase 1, JNK1, PRKM8|
|References||Verheij M, Bose R, Lin XH, et al. (1996) Nature. 380: 75-79.
Verheij M, Ruiter GA, Zerp SF, et al. (1998) Radiotherapy and Oncology. 47: 225-232.
Moriguchi T, Toyoshima F, Masuyama N, et al. (1997) The EMBO J 16: 7045-7053.
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