Phospho-mTOR (Ser2448) (E11) rabbit mAb PE Conjugate
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Product Overview
Description
mTOR, mammalian target of rapamycin, is a Serine/Threonine protein kinase (1-2) that functions as an amino acid and ATP sensor to balance cell growth and nutrient availability (3-4). When sufficient nutrients are available, mTOR transmits a positive signal to p70 S6 kinase and participates in the inactivation of 4E-BP1 (5). mTOR plays a key role in homeostasis and cell growth, and phospho mTOR may be abnormally regulated in tumors. mTOR is a potential target for anti-cancer therapy (6).
Product Details
| Specification | Description |
|---|---|
| Applications | Flow Cytometry |
| Clone | mTORS2448-E11 |
| Format | PE |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser2448 of human phospho mTOR |
| Formulation | 1X PBS, 0.09% NaN3, 0.2% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | 2-8ºC |
| Pseudonyms | Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, FRAP, FRAP1, FRAP2, RAFT1, RAPT1 |
| Uniprot ID | P42345 |
| References | 1. Sabers, C.J. et al. (1995) J. Biol. Chem. 270: 815-822. 2. Brown, E.J. et al. (1994) Nature. 369: 756-758. 3. Gingras, A.C. et al. (2001) Gene. Dev. 15: 807-826. 4. Dennis, P.B. et al. (2001) Science. 294: 1102-1105. 5. Fang, Y. et al. (2001) Science. 294: 1942-1945. 6. Huang, S. and Houghton, P.J. (2003) Curr. Op. Pharmacol. 3: 371-377. |
Images
Flow cytometric analysis of A431 cells treated with phosphatase and unstained as negative control (blue) or treated with phosphatase (red) or EGF (green) and stained using Phospho-mTOR (Ser2448) PE conjugated antibody mTORS2448-E11. Cat. #2377.
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