Phospho-MARCKS (Ser167/170) (C9) rabbit mAb

Catalog #: 2446
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200 uL (0.5 mg/mL)(2446) $475.00
20 uL (0.5 mg/mL)(2446S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description

MARCKS (myristoylated alanine-rich C kinase substrate) is a major PKC substrate expressed in all eukaryotic cells(1,2). It binds to and cross-links actin filaments to serve as a bridge between Ca2+/calmodulin and PKC signaling and attenuates phosphatidylinositol 4,5-bisphosphate plasma membrane signaling (3). MARCKS is involved with cell mobility, phagocytosis, membrane traffic, cell adhesion, and mitogenesis. Ser159, 163, 167 and 170 of MARCKS are phosphorylated by PKC in response to cell groeth and cellular stress (4). MARCKs phosphorylation is believe to induce its tranlocation from plasma membrane to cytoplasm.

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone MARCKSS167170-C9
Format Unconjugated
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, PKCSL, 80 kDa protein light chain, MACS, PRKCSL
Uniprot ID P29966
References 1. El Amri M et al., (2018) J Biomed Sci 25(1):43. doi: 10.1186/s12929-018-0445-
2. Aderem A. (1992) Cell 71:713-6.
3. Hartwig JH, et al., (1992) Nature 356:618-22.
4. Bhat NR. et al., (1991) J Neurisci Res 30: 447-54

Images

Cell Culture Products
Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or treated with staurosporine (red) or with UV+TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 µg/mL. Cat. #2446.
Cell Culture Products
Western blot analysis of NIH3T3 cell extract untreated or treated with TPA using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.1 ug/mL. Cat. #2446.
Cell Culture Products
Flow cytometric analysis of 293T cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 µg/mL. Cat. #2446.
Cell Culture Products
Flow cytometric analysis of 293T cells secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using 0.1 ug/mL of Phospho-MARCKS (Ser167/170) antibody MARCKSS167170-C9 at 0.01 µg/mL (Cat. #2446) or Company C antibody at 0.5 ug/mL (manufacturer's recommended concentration).

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