MARCKS (myristoylated alanine-rich C kinase substrate) is a major PKC substrate expressed in all eukaryotic cells(1,2). It binds to and cross-links actin filaments to serve as a bridge between Ca2+/calmodulin and PKC signaling and attenuates phosphatidylinositol 4,5-bisphosphate plasma membrane signaling (3). MARCKS is involved with cell mobility, phagocytosis, membrane traffic, cell adhesion, and mitogenesis. Ser159, 163, 167 and 170 of MARCKS are phosphorylated by PKC in response to cell groeth and cellular stress (4). MARCKs phosphorylation is believe to induce its tranlocation from plasma membrane to cytoplasm.
Predicted to work with mouse, rat and other homologues.
Detection
Anti-Rabbit IgG
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser167/170 of human phospho MARCKS
Formulation
1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage
-20ºC
Pseudonyms
Myristoylated alanine-rich C-kinase substrate, Protein kinase C substrate, PKCSL, 80 kDa protein light chain, MACS, PRKCSL
1. El Amri M et al., (2018) J Biomed Sci 25(1):43. doi: 10.1186/s12929-018-0445-
2. Aderem A. (1992) Cell 71:713-6.
3. Hartwig JH, et al., (1992) Nature 356:618-22.
4. Bhat NR. et al., (1991) J Neurisci Res 30: 447-54
