Phospho-Lamin A/C (Ser22) (CF12) rabbit mAb APC conjugate

Catalog #: 2439
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100 Tests(2439) $299.00
10 Tests(2439S) $99.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description

Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3) Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone LaminACS22-CF12
Format APC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1
Uniprot ID P02545
References 1. Goldberg, M, et al., (1999) Crit Rev Eukaryot Gene Expr, 9:285-93.
2. Yabuki, M., et ql., (1999) Physiol Chem Phys Med NMR 31: 77-84
3. Rao, L et al., (1996) J Cell Biol 135: 1441:55.
4. Orth K, et al., (1996) J Biol Chem 271:16443-6
5. Nousiainen M, et al., (2006) Proc Natl Acad Sci USA 103:5391-6

Images

Cell Culture Products
Flow cytometric analysis of Hela cells untreated (red) or treated with nocadozole (green) using Phospho-Lamin A/C (Ser22) (CF12) Rabbit mAb (APC Conjugate) LaminACS22-CF12 #2439, or concentration-matched Rabbit (G9) mAb IgG Isotype Control (APC Conjugate) #2144 for cells untreated (black) or treated with nocodazole (blue).

Associated Products