Phospho-Lamin A/C (Ser22) (CF12) rabbit mAb

Catalog #: 2436
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200 uL (0.5 mg/mL)(2436) $475.00
20 uL (0.5 mg/mL)(2436S) $175.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description

Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3). Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).

Product Details

Product Details
Specification Description
Applications Flow Cytometry, WB
Clone LaminACS22-CF12
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1
Uniprot ID P02545
References 1. Goldberg M, et al., (1999) Crit Rev Eukaryot Gene Expr, 9:285-293.
2. Yabuki M, et al., (1999) Physiol Chem Phys Med NMR, 31: 77-84.
3. Rao L, et al., (1996) J Cell Biol, 135: 1441:1455.
4. Orth K, et al., (1996) J Biol Chem, 271:16443-16446.
5. Nousiainen M, et al., (2006) Proc Natl Acad Sci USA, 103:5391-5396.

Images

Cell Culture Products
Flow cytometric analysis of HeLa cells untreated (red) or treated (green) using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 0.01 µg/mL. Cat. #2436 or concentration matched isotope control for untreated (grey) or treated with nocodazole (orange) isotype control Cat. #2141, or negative control (blue).
Cell Culture Products
Western blot analysis of HeLa cell extract untreated or treated with nocodazole using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 10 ng/mL. Cat. #2436.
Cell Culture Products
Flow cytometric analysis of NIH 3T3 cells, secondary antibody only negative control (blue), or untreated (red) or treated with nocodazole (green) using Phospho-Lamin A/C (Ser22) antibody LaminACS22-CF12 at 0.05 µg/mL. Cat. #2436.

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