Phospho-Lamin A/C (Ser22) (CF12) rabbit mAb
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SKU
2436
Lamins are nuclear membrane proteins that are involved with cell cycle control, chromatin organization, and DNA replication (1,2). Upon caspase 6 cleavage, lamin A/C serves as a molecular marker for capspase 6 activity. Lamin A/C is cleaved during cellular apoptosis into a large (41-50 kDa) as well as a small (28 kDa) fragment leading to nuclear dysfunction and cell death (3). Phosphorylation of lamin A/C at Ser32 by MAPK/CDK signals cell cycle progression and mitosis (4,5).
| Applications | Flow Cytometry, WB |
|---|---|
| Clone | LaminACS22-CF12 |
| Format | Unconjugated |
| Validated Reactivity | Human, Mouse |
| Cross Reactivity | Predicted to work with mouse, rat and other homologues. |
| Detection | Anti-Rabbit IgG |
| Clonality | Monoclonal |
| Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser22 of human phospho Lamin A/C |
| Formulation | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA |
| Isotype | Rabbit IgGk |
| Preparation | Protein A+G |
| Recommended Usage | 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information. |
| Storage | -20ºC |
| Pseudonyms | Prelamin-A/C, 70 kDa lamin, Renal carcinoma antigen NY-REN-32, LMNA, LMN1 |
| Uniprot ID | P02545 |
| References | 1. Goldberg M, et al., (1999) Crit Rev Eukaryot Gene Expr, 9:285-293. 2. Yabuki M, et al., (1999) Physiol Chem Phys Med NMR, 31: 77-84. 3. Rao L, et al., (1996) J Cell Biol, 135: 1441:1455. 4. Orth K, et al., (1996) J Biol Chem, 271:16443-16446. 5. Nousiainen M, et al., (2006) Proc Natl Acad Sci USA, 103:5391-5396. |
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