Phospho-HS1 (Tyr397) (F12) rabbit mAb APC Conjugate

From $118.80
In stock
Only %1 left

HS1 is expressed in lymphoid and hematopoietic cells, and is heavily post-translationally modified. HS1 deficient mouse models have demonstrated the protein's role in receptor-mediated apoptosis and proliferation. HS1 is phosphorylated at Tyr378 and Tyr397 by the kinase Syk, providing a high-affinity binding site for SH2 domains from the Src family. Following this interaction, HS1 is then phosphorylated at Tyr222 by c-Fgr, Lyn, and Fyn kinases. HS1 plays an important role in T cell signaling, where HS1 phosphorylation recruits and activates Vav1 at the immune synapse. As a homolog of the actin binding protein cortactin, HS1 has been shown to mediate neutrophil chemotaxis through phosphorylation of tyrosines 222, 378, and 397.

More Information
Applications Flow Cytometry
Clone HS1Y397-F12
Format APC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr397 of human phospho HS1
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1
Uniprot ID P14317
References Brunati AM, et al., (1999) Journal of Biological Chemistry. 274:7557-7564. Cavnar PJ, et al., (2012) Journal of Biological Chemistry. 287: 25466-25477.
Write Your Own Review
You're reviewing:Phospho-HS1 (Tyr397) (F12) rabbit mAb APC Conjugate
Your Rating