Phospho-Chk2 (Thr68) (D12) rabbit mAb FITC conjugate

Catalog #: 2118
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100 Tests(2118) $299.00
10 Tests(2118S) $99.00
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Note

20 μL is enough antibody for at least 20 Western blots.

Product Overview

Description

Checkpoint kinase 2 (Chk2) plays a major role in the checkpoint response to DNA damage. Chk2 is initially inactive in its monomeric, unphosphorylated form. Phosphorylation at Thr68 induces homodimerization, initiating autophosphorylation within the kinase loop at Ser516 and phorphorylation events within the auto-inhibitory loop at Thr383 and Thr387. After these phosphorylations, active dimers and monomers can then phosphorylate substrates such as Cdc25C and BRCA1. In humans, Chk2 genetic deletion and missense variants have been found to be associated with increased risk of breast and colon cancer. Constitutively phosphorylated Chk2 at Thr68 has been found in many human cancer cell lines, especially ones with mutations in p53.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone Chk2T68-D12
Format FITC
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phosphor-peptide corresponding to residues surrounding to Thr68 of human phospho Chk2
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Serine/threonine-protein kinase Chk2, Cds1 homolog, Hucds2, CHEK2, RAD53
Uniprot ID O96017
References Ahn J, Urist M, and Prives C. (2004) DNA Repair. 3: 1039-1047.

Images

Cell Culture Products
Flow cytometric analysis of Hela cells treated with lambda phosphatase (red) or treated with UV+TPA (green) using Phospho-CHK2 (Thr68) (D12) Rabbit mAb (FITC Conjugate) CHK2T68-D12 #2118, or concentration-matched Rabbit (G9) mAb IgG Isotype Control (FITC Conjugate) #2143 for cells treated with lambda phosphatase (black) or treated with UV+TPA (blue).

Associated Products