c-FOS belongs to the Fos family of nuclear oncogenes which include Fos B, Fos-related antigen 1 (FRA1), Fos-related antigen 2 (FRA2) in addition to c-Fos (1). Activator Protein-1 (AP-1) is formed upon dimerization of Fos proteins with Jun protiens (c-Jun, Jun B, and JunD) (2,3). AP-1 is considered a transcription factor that binds to TRE/AP-1 elements and activates transcription. ERK5 is involved with c-Fos phosphorylation at Ser32 and Thr232 which increase c-Fos stability and its nuclear translocation.
Predicted to work with mouse, rat and other homologues.
Clonality
Monoclonal
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Ser32 of human phospho c-Fos
Formulation
1X PBS, 0.09% NaN3, 0.2% BSA
Isotype
Rabbit IgGk
Preparation
Protein A+G
Recommended Usage
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
