Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb

Catalog #: 2386
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200 uL (0.5 mg/mL)(2386) $570.00
20 uL (0.5 mg/mL)(2386S) $210.00
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Product Overview

Description
Aurora kinases (serine/threonine kinases) are essential requirement for the onset and progression of mitosis. These kinases share a similar protein structure as well as kinase activity, however each kinase display distinct cellular and subcellular localization. Each Aurora member is phosphorylated at specific residues upon co-factor binding during mitosis. Aurora kinases acquire active kinase conformations due to the activation loop. The active kinase conformation is acquired upon auto-phosphorylation through an intermolecular (trans)-reaction within Aurora kinase domain. Aurora Kinase A (Aurora A) is involved in G2/M transition. AuroraA promotes centrosome maturation and mitotic spindle assembly, whereas AuroraB and AuroraC act as chromosome-passenger complex proteins. They play a crucial role in chromosomal binding to kinetochores and segregation of chromosomes. Aurora B is widely distributed in the cell, while AuroraC is expressed mainly in the meiotically-active germ cells. Aurora kinases are auto-phosphorylated into active forms at conserved threonine residues (i.e. the Thr288 (AurA), Thr232 (AurB) and Thr195 (AurC) residues). AuroraA auto-phosphorylation is initiated by several co-factors acting at different steps of mitosis. AroraB and AruroaC auto-phosphorylation are mediated by survivin and Borealin proteins.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone AuroraABC-CC12
Format Unconjugated
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Aurora kinase A/B/C, AURKA, AURKB, AURKC
Uniprot ID O14965 Q96GD4 Q9UQB9
References Hochegger H, et al., (2013) Open Biol. 3:120185.
Carmena M, et al., (2009) Curr Opin Cell Biol 21:796–805. 
Bolanos-Garcia VM. (2005) Int J Biochem Cell Biol. 37:1572–1577.
Kimmins S, et al., (2007) Mol Endocrinol. 2007;21(3):726–739.
Vader G, and Lens SMA. (2008) Biochim Biophys Acta. 1786:60–72.

Images

Cell Culture Products
Flow cytometric analysis of HeLa cells secondary antibody only negative control (blue) or untreated (grey) or treated with nocodazole (orange) using 0.5 µg/mL of isotype control Cat. #2141 or untreated (red) or treated (green) using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 µg/mL. Cat. #2386.
Cell Culture Products
Peptide blocking flow cytometric analysis of HeLa cells unstained cells negative control (light blue) or untreated (red) or treated with nocodazole (green) or untreated and blocked with phospho-peptide (black) or nocodazole and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or nocodazole and blocked with non-phospho peptide (purple) using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) antibody AuroraABC-CC12 at 0.5 µg/mL. Cat. #2386.

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