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Phospho-4E-BP1 (Thr37/46) (A5) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||Eukaryotic translation initiation factor 4E-binding protein 1 (PHAS-1, EIF4EBP1, eIF4E-binding protein 1, phospho 4E-BP1) is one member of a family of translation repressor proteins encoded by the EIF4EBP gene (1). The protein directly inhibits cap-dependent translation by binding eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multi-subunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of cap-dependent mRNA translation(2). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of phospho 4E-BP1 to eIF4E, it is thought to prime phospho 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.|
|Applications||Flow Cytometry, WB, IHC|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Thr37/46 of human phospho 4E-BP1.|
|Validated Reactivity||Human, Mouse, Rat|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|References||1. Pause A, et al. (1994) Nature 371: 762-7
2. Mader S, et al. (1995) Mol. Cell. Biol. 15: 4990–7.
Immunohistochemical staining of rat kidney cells including peptide blocking shows phospho-specificity for 4EB1T37T46-A5 Cat. #2041. Anti-rabbit secondary antibody staining (red) is punctate in the nuclei. Neither control peptide (A) nor non-phospho 4E-BP1 peptide (B) blocks 4EB1T37T46-A5 staining but phospho 4E-BP1 (T37/T46) peptide efficiently blocks signal (C). No signal was observed when the primary antibody is omitted (D). The central object in the field is a glomerulus with surrounding tubules sectioned in various orientations.
Western blot analysis of K562 cell extract untreated or treated with imatinib using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL. Cat. #2041.
Western blot analysis of NIH3T3 cell extract untreated or treated with PDGF using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 1 ng/mL. Cat. #2041.
Flow cytometric analysis of C6 cells untreated cells stained with secondary antibody as negative control (blue) or untreated (red) or treated with UV and TPA (green) and stained using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1µg/mL. Cat. #2041.
Peptide blocking flow cytometric analysis of Jurkat cells LY294002 plus wortmannin plus U0126 LWU) treated cells stained with secondary antibody as negative control (light blue) or treated with LWU (red) or treated with FBS (green) or LUW and blocked with phospho-peptide (black) or FBS and blocked with phospho peptide (gold) or LUW and blocked with non-phospho peptide (dark blue) or FBS and blocked with non-phospho peptide (purple) using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1µg/mL. Cat. #2041.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or treated with LY294002 + U0126 + wortmanmin (red) or with FBS (green) using 0.1 ug/mL of Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 (Abwiz Cat. #2041) or Company C antibody at 0.5 ug/mL (manufacturer's recommended concentration).
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