SARS-CoV-2 Epsilon 427 Variant Recombinant Spike Trimer His Tag

Catalog #: 2591
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1mg(2591M) $1440.00
200 uL (0.5 mg/mL)(2591-200) $570.00
20 uL (0.5 mg/mL)(2591-20) $210.00
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Short Description

Coronaviruses belong to a large family of protein enveloped positive-strand RNA viruses that are known to cause upper respiratory, gastrointestinal, central nervous system diseases in animals and human (1). One of the main proteins on viral envelop is a heavily glycosylated spike (S) protein (2). Trimeric S protein is involved in host receptor angiotensin-converting enzyme 2 (ACE2) recognition and mediates viral entry into cells. It is the principle antigenic determinant of neutralizing antibodies (3). S protein is recognized by humoral immune response during infection leading to inflammatory response. Homotrimeric S protein is a class I fusion protein that forms large protrusions from the virus surface and undergoes a significant structural rearrangement to fuse the viral membrane with the host-cell membrane once it binds to the host receptor (4).

Product Details

Product Details
Specification Description
Applications ELISA
Clone COV2-Epsilon427
Format His tag
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-SARS-CoV-2 mAb
Clonality SARS-CoV-2
Formulation 1X PBS, 0.09% NaN3
Isotype SARS-CoV-2
Preparation His tag purification
Recommended Usage Control antigen for SARS-CoV-2 (COVID-19) diagnostic assays. Contains C-terminal His tag used for purification.
Storage 2-8ºC
Pseudonyms Severe acute respiratory syndrome coronavirus 2 spike protein, 2019-nCoV spike protein
Uniprot ID XXXXXX
References 1.Walls A.C. et al., Cell 2019, 176: 1026-1039.
2.Tang T. et al., 2020, Antiviral Res., 178:10479.
3.Jiang S et al., 2022, Trends Immunol, 41:355-359.
4.Bosch BJ, et al., 2003, J Virol 77:8801-8811.

Images

Cell Culture Products
Concentration–response curves for binding of CoV2 spike protein to human ACE2 in cell-free ELISA-type assays. Microtiter wells were coated with 100 uL of each spike trimer at 2 ug/mL in PBS at 4˚C overnight. The wells were washed with PBS and blocked with 200 µL of 1% BSA/PBS. ACE2-Fc was serially diluted from 2 µg/mL in 1% BSA/PBS. The blocker was discarded, and the wells were incubated with 100 µL of serially diluted ACE2-Fc at 37˚C for 1 hour. The wells were washed with PBS and the bound ACE2-Fc was detected with 100 µL of Peroxidase AffiniPure Goat Anti-Human IgG, Fc fragment specific (Jackson Immuno Research 109-035-098)(1:5,000 in 1% BSA/PBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at RT for 5 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek).
Cell Culture Products
SARS-CoV-2 spike protein variant purity by SDS-PAGE. Separation of SARS-CoV-2 spike recombinant protein (8xHis-Tag, 2 ug) reduced (R) and non-reduced (NR) condition followed by Coommassie blue staining shows >90% purity.

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