SARS-CoV2 G10xA1 human neutralizing mAb

Catalog #: 2571
Select Your Size Price
200 uL (0.5 mg/mL)(2571-200 uL (0.5 mg/mL)) $475.00
20 uL (0.5 mg/mL)(2571-20 uL (0.5 mg/mL)) $175.00
From $175.00

Need to make a custom order?

Contact us

Note

20 μL is enough antibody for at least 20 Western blots. 

Product Details

Product Details
Specification Description
Special Notice Avoid multiple freeze and thaw cycles
Applications Functional Assay, ELISA
Clone G10xA1
Format Unconjugated
Validated Reactivity Human
Cross Reactivity No
Detection Anti-SARS-CoV-2 NP mAb
Clonality Monoclonal
Immunogen A recombinant SARS-CoV-2 spike protein containing RBD.
Formulation 1X PBS, 0.2% BSA
Isotype Human IgG1k
Preparation Protein A
Recommended Usage A recombinant SARS-CoV-2 spike protein containing RBD.
Storage -20ºC
Uniprot ID XXXXXX

Images

Cell Culture Products
Microtiter wells were coated with 100 µL of ACE2-Fc (Cat# 2566) at 2 µg/mL in PBS at 4˚C overnight. The wells were washed with 1XPBS and blocked with 200 µL of 1% BSA/1XPBS. The antibody (Gx10A15 #2571 was titrated from 1 µg/mL in 1% BSA/1XPBS and mixed with equal volume of spike trimer Avi-tag of each variant (Acrobiosystems). The blocker was discarded, and the wells incubated with 100 µL of the mixture of antibody and the spike trimer at 37˚C for 1 hour. The wells were washed with 1XPBS and the bound spike trimer was detected with 100 µL of High Sensitivity Streptavidin-HRP (Thermo Fisher 21130) (1:5,000 in 1% BSA/1XPBS) at 37˚C for 1 hour. The wells were washed with PBS and the wells were developed with 100 µL of MB/E Ultra Sensitive, Blue, Horseradish Peroxidase Substrate (EMD Millipore ES022-500ml) at 37˚C for 15 min. The reaction was stopped with 100 µL of 0.6N H2SO4 and the signals were read at 450 nm using a plate reader (Biotek). The experiments were performed in triplicates and the average was used to calculate % inhibition and IC50 (ng/mL) for each variant.

Associated Products