Phospho-PKC (pan) (gamma Thr514) (PF4) rabbit mAb FITC conjugate

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100 Tests(2453) $299.00
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Product Overview

Protein Kinase (PKC) is a 12 member family of serine/threonine kinases termed conventional or classical  (a b, g), novel (d,e,and q), atypical (zl) and PKN and PKC-related (PKN1, PKN2 and PKN3) playing significant role in several signaling processes involved in physiological and pathological setting (1). PKC activation translates into gene expression modulation, cell division, migration, proliferation, differentiation, and cell survival and apoptosis (2).  PKC members are classified based on their distinct cofactor requirements and the extent of homology between their regulatory elements (3,4). PKCalpha, beta I, beta II, and gamma constitute the conventional PKC isoforms, characterized by the presence of two cysteine-rich zinc finger domains, C1a and C1b (5,6) which bind to diacylglycerol (DAG) and phosphatidylserine (PS) (7). In addition, PKC contains a C2 domain, responsible for binding anionic phospholipids like phosphatidylinositol bisphosphate (PIP2) in a Ca2+-dependent manner (8,9). The atypical PKC, PKCz andl share ATP-binding domain and the catalytic domain with PKC. They contain a single C1 domain that lacks residues necessary for binding DAG (10).

 Association of PKCg to the membrane enables a conformation that permits phosphoinositide-dependent protein kinase1 (PDK-1) (11) to bind and phosphorylate a site in the activation loop, Thr514. (12,13). Thr514 phosphorylation leads to a conformation change enabling phosphorylation of at two carboxyl-terminal sites namely, the turn motif and hydrophobic motif, as a result of which the fully phosphorylated conventional PKC is released from the membrane, and positioned in the cytoplasm as an inactive form (14,15). Binding of Ca2+ induces low-affinity interaction with the membrane, whereas the membrane imbedded cofactor DAG to PKC results in high-affinity interaction of PKC with the membrane (16). 

Product Details
More Information
Applications Flow Cytometry
Clone PKCgT514-PF4
Format FITC
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Thr514 of human phospho PKCγ
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Protein kinase C gamma type, PRKCG, PKCG
Uniprot ID P17252
References 1. Mugami S, et al., 2018, Mol. Cell. Endocrinol. 463:97-105.
2. Isakov N, 2018, Semin. Cancer Biol. 48:36-52.
3. Mellor H, and Parker PJ, 1998, Biochem. J. 332:281-92.
4. Newton AC, 1995, J. Biol. Chem. 270:28495-8.
5. Ho C, et al., 2001, Biochem. 40:10334-41.
6. Hurley JH, et al., 1996, Protein Sci. 6:477-80.
7. Colon-Gonzalez F, and Kazanietz MG, 2006, Biochim. Biophys. Acta 1761 :827-37.
8. Naleski EA, and Falke JJ, 1996, Protien Sci, 5 :2375-90.
9. Orr JW, and Newton AC, 1992, Biochem 31:4667-4673.
10. Antal CE and Newton AC, 2014, Biochem Soc. Trans. 42:1477-1483.
11. Sonnenberg ED, et al., 2001 J Biol Chem, 276:45289-97.
12. Dephoure N, et al., 2008, Proc. Natl. Acad Sci. U.S.A. 105:10762-67.
13. Barnett ME, et al., 2007, Cell Signal. 19:1820-9.
14. Newton AC, 2003, Biochem. J. 370 :361-71.
15. Parekh DB, et al., 2000, EMBO J. 10:496-503.
16. Edwards AS, et al., 1999, J Biol. Chem., 274:6461-8.
Cell Culture Products
Flow cytometric analysis of HT1080 cells treated with staurosporine (red) or untreated (green) using Phospho-PKCg (Thr514) (PF4) Rabbit mAb (FITC Conjugate) PKCgT514-PF4 #2453, or concentration-matched Rabbit (G9) mAb IgG Isotype Control (FITC Conjugate) #2143 for cells treated with staurosporine (black) or untreated (blue).