Phospho-Lyn (Tyr507) (5B6) rabbit mAb

Catalog
2416
Select Your Size Price
200 uL (0.5 mg/mL)(2416) $475.00
20 uL (0.5 mg/mL)(2416S) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

Lyn, along with Btk, supports the abnormal growth and survival of neoplastic mast cells. Phosphorylated Lyn has been identified in these cancerous cells, along with phosphorylated Btk, Hck, and Stat5. Dasatinib, a chemotherapy drug used to treat leukemia, is a tyrosine kinase inihibitor that binds directly to Lyn in neoplastic cells. Lyn and Btk have also been shown to be involved in IgE receptor-dependent activation. Increased Lyn activity, detected by higher amounts of phospho Lyn, has been demonstrated in breast cancer cell lines. This is likely mediated through effects of upstream regulators of Lyn, rather than mutations in Lyn itself.

Product Details
More Information
Applications Flow Cytometry, WB
Clone LynY507-5B6
Format Unconjugated
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr507 of human phospho Lyn
Formulation 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage 1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage -20ºC
Pseudonyms Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, JTK8
Uniprot ID P07948
References Gleixner KV, Mayerhofer M, Cerny-Reiterer S et al. (2011) Blood. 28: 1077-1086.
Choi Y, Bocanegra M, Kwon MJ et al. (2010) Molecular and Cellular Pathology. 70: 2296-2306.
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01µg/mL. Cat. #2416.
Cell Culture Products
Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01µg/mL. Cat. #2416.
Cell Culture Products
Flow cytometric analysis of C2C12 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using Phospho-Lyn (Tyr507) antibody LynY507-5B6 at 0.01µg/mL. Cat. #2416.
Cell Culture Products
Western blot analysis of extracts of HeLa cells treated with H2O2. Cell lysates were ran on a SDS-PAGE gel, transferred to nitrocellulose membrane, blocked and non-treated (-) or treated with lambda phosphatase (+) and stained using anti-phosph-Lyn (Tyr507) 5B6 rabbit recombinant antibody #2416.
Cell Culture Products
Western blot analysis of Hela cell extract, untreated or treated with H2O2 using Phospho-LYN(TYR507) antibody LYNY507-5B6 at 0.01 ug/mL. Cat. #2416.
Cell Culture Products
Western blot analysis of HeLa cell extract, untreated or treated with H2O2 using 0.01 µg/mL Phospho-Lyn (Tyr507) antibody LynY507-5B6 Cat. #2416 or Company A antibody at 1 µg/mL (manufacturer’s recommended concentration) developed using the same exposure.