Phospho-c-Cbl (Tyr700) (E1) rabbit mAb FITC conjugate

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2323
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100 Tests(2323) $299.00
10 Tests(2323S) $99.00
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Product Overview
Description

The c-Cbl (Casitas B-lineage Lymphoma) proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that contains multiple functional domains, including an amino-terminal tyrosine kinase-binding (TKB) domain, a RING finger motif, and a proline-rich region. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) and on other nonreceptor tyrosine kinases, while the RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs (1). The proline-rich region contains 14-3-3 protein-binding and SH3 domain-binding motifs. c-Cbl is phosphorylated at Y700, Y731, and Y774 by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for immunoglobulins, antigens, hormones, growth factors, and cytokines. Phosphorylated Y774 interacts with the SH2 domain of Crk (1,2). The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells (3,4). Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5).

Product Details
More Information
Applications Flow Cytometry
Clone CblY700-E1
Format Unconjugated
Validated Reactivity Human, Mouse, Rat
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr700 of human phospho c-Cbl
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms E3 ubiquitin-protein ligase CBL, Casitas B-lineage lymphoma proto-oncogene, Proto-oncogene c-Cbl, RING finger protein 55, RING-type E3 ubiquitin transferase, Signal transduction protein CBL, CBL2, RNF55
Uniprot ID P22681
References 1. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2: 294-307.
2. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273: 8323-8331.
3. Blake, T.J. et al. (1991) Oncogene. 6: 653-657.
4. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76: 473-482.
5. Kamei, T. et al. (2000) Int. J. Oncol. 17: 335-339.
Images
Cell Culture Products
Flow cytometric analysis of C6 cells cell treated with imatinib and unstained as negative control (blue) or treated with imatinib (red) or with pervanadate (green) and stained using Phospho-c-Cbl (Tyr700) FITC conjugated antibody CblY700-E1. Cat. #2323.