NOD1 (G7) rabbit mAb

Catalog
2526
Select Your Size Price
200 uL (0.5 mg/mL)(2526-200 uL (0.5 mg/mL)) $475.00
20 uL (0.5 mg/mL)(2526-20 uL (0.5 mg/mL)) $175.00
$175.00
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Note

20 μL is enough antibody for at least 20 Western blots. 

Product Overview
Description

Human nucleotide-binding oligomerization domain containing protein (NOD) 1 belongs to the NOD-like receptor family, which consists of pattern recognition receptors (PRRs) that reside in the cytoplasm of cells (1,2). NOD1 is ubiquitously expressed and recognizes bacterial peptidoglycan fragments, including d-glutamyl-meso-diaminopimelic acid found in gram-negative and some gram-positive bacteria (1-3). NOD1 contains three domains: a N-terminal caspase recruitment domain responsible for mediating downstream inflammatory signaling cascades, a nucleotide binding domain necessary for receptor oligomerization and activation, and a leucine-rich C-terminal region that recognizes conserved microbial patterns and ligands (1, 3). Upon interaction with its ligands, NOD1 undergoes self-oligomerization to acquire an active conformation that elicits downstream signaling of proinflammatory and stress pathways (4,5). The adaptor protein receptor-interacting protein kinase-2 (RIPK2) then associates with the caspase recruitment domain motif of NOD1 and subsequently recruits the kinase TAK1 as a prerequisite to activate nuclear factor κB (NF-κB) and MAPK signaling (4-7). The NF-κB and MAPK cascades integrate signaling by NOD1, NOD2, and other innate immune complexes such as TLRs and various inflammasomes to vary the intensity, duration, and pattern of the downstream outcomes. NOD1 signaling can potentiate or be additive to that of TLR4 (8,9).

Product Details
More Information
Applications WB
Clone NOD1-G7
Format Unconjugated
Validated Reactivity Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen Synthetic peptide
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Human nucleotide-binding oligomerization domain containing protein (NOD) 1
Uniprot ID Q9Y239
References 1. Correa RG, et al., 2012, Biosci Rep, 32:597–608.
2. Moreira LO, and Zamboni DS. 2012, Front Immunol, 3:328.
3. Mogensen TH. 2009, Clin Microbiol Rev, 22:240–273.
4. Caruso R, et al., 2014, Immunity. 2014, 41:898–908.
5. Strober W, et al., 2006, Nat Rev Immunol, 6:9–20.
6. Kobayashi K, et al., 2002, Nature, 416:194–199.
7. Nembrini C, et al., 2009, J Biol Chem, 284:19183–19188.
8. Tang D, et al., 2012, Immunol Rev, 249:158–175.
9. Chan KL, et al., 2017, Cell Reports, 18:2415–2426.
Images
Cell Culture Products
Western blot analysis of (1) mice wild type (WT) splenocytes, (2) mice NOD1KO splenocytes (3) HEK293 Mock and (4) HEK293-mouse NOD1 using NOD1 rabbit mAb, NOD1-G7, Cat#2526.