Phospho-Stat5 (Tyr694) (G11) rabbit mAb PE conjugate

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Product Overview
Description Stat5 activation occurs in response to many ligands including prolactin, IL-2, growth hormone, and GM-CSF. Tyr694 phosphorylation is obligatory activation of Stat5 (1,2), and is mediated by Src upon erythropoietin stimulation (3). Phospho Stat5 is constitutively active in some leukemic cell types (4), and phospho Stat5 is found in some endothelial cells when treated with IL-3, suggesting its involvement in cell motility and angiogenesis (5). Stat5 has been shown to be encoded by two separate genes, Stat5a and Stat5b, which share over 90% amino acid sequence identity. In different cell types, Stat5a and Stat5b are independently regulated and activated. For example, interferon treatment predominantly activates Stat5a in U937 cells and Stat5b in HeLa cells (6).
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Applications Flow Cytometry
Clone Stat5Y694-G11
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr695 of human phospho Stat5
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
Storage 2-8ºC
Pseudonyms Signal transducer and activator of transcription 5A, STAT5A, Signal transducer and activator of transcription 5B, STAT5B
Uniprot ID P42229
References 1. Gouilleux, F. et al. (1994) EMBO J. 13:4361-4369.
2. Wakao, H. et al. (1994) EMBO J. 13:2182-2191.
3. Okutani, Y. et al. (2001) Oncogene. 20:6643-6650.
4. Demoulin, J.B. et al. (1999) J. Biol. Chem. 274:25855-25861.
5. Dentelli, P. et al. (1999) J. Immunol. 163:2151-2159.
6. Meinke, A. et al. (1996) Mol. Cell. Biol. 16:6937-6944.
Cell Culture Products
Flow cytometric analysis of NIH3T3 cells unstained and treated with imatinib as negative control (blue) or stained and treated with imatinib (red) or treated with pervanadate (green) using Phospho-Stat5 (Tyr694) antibody Stat5Y694-G11 PE conjugate. Cat. #23299.