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Rabbit Monoclonal Antibody Development

Rabbit Monoclonal Antibody Development

Recombinant rabbit monoclonal antibodies have historically remained elusive due to intellectual property and technological barriers. Rabbit antibodies have unique advantages that make them ideal for research or diagnostic uses or as sources of therapeutic lead candidates. Due to higher diversity within antigen binding regions (CDRs), rabbit antibodies created from immunized sources have higher affinity compared to their mouse counterparts. Rabbit antibodies typically possess picomolar KD binding affinities, making affinity maturation unnecessary.

Rabbit monoclonal antibodies are typically derived from hybridomas, which are commonly poorly characterized and can exhibit high batch-to-batch variability. Hybridoma library screening is also limited (104-105), often missing rare clones that recognize important, less immunodominant epitopes. Abwiz Bio has solved these issues through a proprietary recombinant rabbit monoclonal antibody development platform, RabWizTM.

The unique advantages of RabWizTM include:

  • A proprietary phage display vector compatible with unmodified rabbit Fabs

    • The unique disulfide architecture of rabbit Fabs is known to cause toxicity in E. coli, which degrades the quality of phage libraries. Others have used sub-optimal systems that restrict usage of the full rabbit immune repertoire. Our highly optimized Fab-phage display vector expresses rabbit Fabs without modification.

  • A primer set that covers maximal VH gene diversity

    • Through iterative improvement after creating dozens of rabbit libraries, we have created a primer set that covers the highest reported rabbit VH sequence diversity to date, including multiple FR1 sequences that are not currently in publicly available databases. This ensures that the entire rabbit immune repertoire is isolated during the cloning process.

  • WizAmpTM cloning (US 9,890,414)

    • In our patented WizAmp™ procedure (US 9,890,414), a single non-gene-specific primer is used to amplify antibody genes prior to digestion and cloning into the library vector. This enables uniform amplification of antibody genes, allowing creation of unbiased, high quality libraries.

 

Partnership opportunities

Abwiz Bio is pleased to perform contract work and to engage in co-development partnerships with a variety of research institutions and pharmaceutical companies. Abwiz Bio offers a variety of services that leverage our world-class, proprietary technology platform. Our partnership structure is highly flexible, and we are excited and interested in pursuing partnerships with pharmaceutical companies, research institutes or individual academic researchers. Call or e-mail us today to see how we can help you achieve your goals.

 
  • Recombinant monoclonal antibody development contracts

  • Co-development projects

  • Fee-for-service arrangements

 

Phage display based antibody generation

RabWiz™ employs Abwiz Bio’s proprietary Needle-in-a-Haystack™ phage display technology and patented antibody library cloning method WizAmp™. This combines conventional animal immunization protocols with the robustness of phage display. These together enable the generation of antibodies with affinities typically attained only through hybridoma development, but doing so with the speed and diversity of phage display technology. The process allows for efficient, high throughput identification of rare binders with distinctive characteristics required for a specific application. Using this system, Abwiz Bio can create antibodies that recognize targets that have traditionally been difficult to attain, such as GPCRs and ion channels.

High affinity rabbit antibodies can be created in less than 6 months from the date of rabbit immunization. Abwiz Bio’s monoclonal antibody development platform is coupled with our friendly business terms (no royalties and minimal upfront fee).

 

Project development timeline

Step I - Rabbit Immunization

 

Activity Time Frame
Ia - Antigen preparation Depends on target
Ib - Rabbit immunization ~ 2 months
   

Step II - Library Construction

 

Activity Time Frame
Library Construction ~3 weeks

 

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Step III - Phage Selection

 

Activity Time Frame
IIIa - Panning/screening
  • Fab ELISA screening data deliverable
  • CDR sequencing data deliverable
  • Affinity measurement deliverable (optional)
~2 weeks
IIIb - Epitope masking selection (optional)
  • Repeat panning to obtain new clones
~2 weeks

 

Created by potrace 1.10, written by Peter Selinger 2001-2011

Step IV - Recombinant Antibody Small-Scale Production

 

Activity Time Frame
Recombinant Antibody Small-Scale Production
  • IgG antibody ELISA data deliverable
  • ~1 mg antibody deliverable
~5 weeks

 

Created by potrace 1.10, written by Peter Selinger 2001-2011

Step V - Recombinant Antibody Large-Scale Production

 

Activity Time Frame
Recombinant Antibody Large-Scale Production
  • >3 mg antibody deliverable
~5 weeks

 

Project Completion

 

Activity Time Frame
Total time to create a new high-affinity recombinant rabbit antibody ~7 months

 

Rabbit recombinant monoclonal antibodies represent a novel source of untapped potential and make great tools for use as research reagents or for high sensitivity immuno-detection in diagnostic assays. Rabbit antibodies are emerging as a unique source of lead candidates for therapeutic applications. Abwiz Bio has partnered with experts in rabbit antibody humanization, and our recombinant monoclonal antibody development platform is ideal for engineering humanized variants after the initial lead identification.