Phospho-Jak1 (Tyr1022/1023) (F11) rabbit mAb PE Conjugate

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2412
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100 Tests(2412) $299.00
10 Tests(2412S) $99.00
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Product Overview
Description

Jak1 plays an essential role in the IFN-α and IFN-γ response pathways and is tyrosine-phosphorylated upon cellular exposure to these signals. Jak1 oral inhibitors have been used to benefit patients with advanced myelofibrosis, where Jak1 was initially shown to be constitutively active in the peripheral blood cells of these patients. Targeted, small-molecule Jak inhibitors have also been used for treatment of rheumatoid arthritis. In cases of advanced melanoma, acquired resistance to PD-1 blockade drugs is associated with loss-of-functions of mutations in Jak1/2 genes. These mutations block interferon gamma signaling and prevent programmed death ligand 1 (PD-L1) expression in tumor cells.

Product Details
More Information
Applications Flow Cytometry
Clone Jak1Y10221023-F11
Format PE
Validated Reactivity Human, Mouse
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Tyr1022/1023 of human phospho-Jak1
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgG1k
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Tyrosine-protein kinase JAK1, Janus kinase 1
Uniprot ID P23458
References Muller M, Briscoe J, Laxton C et al. (1993) Nature. 366: 129-135.
Verstovesk S, Kantarjian H, Mesa RA et al. New England Journal of Medicine. 363: 1117-1127.
Shin DS, Zaretsky JM, Escuin-Ordinas H et al. (2017) Cancer Discovery. 7:188-201.
Boyle DL, Soma K, Hodge J, et al. (2014) Annals of the Rheumatic Diseases. 74: 1311-1316.
Images
Cell Culture Products
Flow cytometric analysis of Jurkat cells untreated and unstained as negative control (blue) or untreated (red) or treated with IFNα + IL-4 + pervanadate (green) and stained using Phospho-Jak1 (Tyr1022/1023) PE conjugated antibody Jak1Y10221023-F11 . Cat. #2412.