Phospho-Histone H3 (Ser10) (4B6) rabbit mAb FITC conjugate

Catalog #: 2063
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100 Tests(2063) $299.00
10 Tests(2063S) $99.00
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20 μL is enough antibody for at least 20 Western blots.

Product Overview


Histones are highly conserved proteins that serve the core of nucleosomes, which serve to organize chromatin fiber for DNA packing. Histone H3 phosphorylation plays a major role in both transcriptional activation, which requires unpacking of the chromatin structure, and in chromosome packing during cell division. Histone H3 is phosphorylated at residues Ser10 and Ser28, and is acetylated at Lys14. Phosphorylation at Ser10 occurs during entry into mitosis prior to chromatin condensation, and phosphorylation at Ser28 follows a similar pattern. In response to EGF stimulation, it has been proposed that sequential Ser10 phosphorylation, then Lys14 acetylation occurs, causing a change in chromatin structure and gene activation.

Product Details

Product Details
Specification Description
Applications Flow Cytometry
Clone HisH3S10-4B6
Format FITC
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding Ser10 of human phospho histone H3
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Histone H3.1t, H3t, H3FT, HIST3H3
Uniprot ID Q16695
References 1. Hans F and Dimitrov S. (2001) Oncogene. 20: 3021-3027.
2. Cheung P, Tanner KG, Cheung WL, et al. (2000) Molecular Cell. 5: 905-915.


Cell Culture Products
Flow cytometric analysis of Hela cells unstained as negative control (blue) or untreated (red) or treated with nocodazole (green) and stained using Phospho-Histone H3 (Ser10) FITC conjugated antibody HisH3S10-4B6. Cat. #2063.

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