Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) (CC12) rabbit mAb APC conjugate

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2389
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100 Tests(2389) $299.00
10 Tests(2389S) $99.00
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Product Overview
Description

Aurora kinases (serine/threonine kinases) are essential requirement for the onset and progression of mitosis. These kinases share a similar protein structure as well as kinase activity, however each kinase display distinct cellular and subcellular localization. Each Aurora member is phosphorylated at specific residues upon co-factor binding during mitosis. Aurora kinases acquire active kinase conformations due to the activation loop. The active kinase conformation is acquired upon auto-phosphorylation through an intermolecular (trans)-reaction within Aurora kinase domain. Aurora Kinase A (Aurora A) is involved in G2/M transition. AuroraA promotes centrosome maturation and mitotic spindle assembly, whereas AuroraB and AuroraC act as chromosome-passenger complex proteins. They play a crucial role in chromosomal binding to kinetochores and segregation of chromosomes. Aurora B is widely distributed in the cell, while AuroraC is expressed mainly in the meiotically-active germ cells. Aurora kinases are auto-phosphorylated into active forms at conserved threonine residues (i.e. the Thr288 (AurA), Thr232 (AurB) and Thr195 (AurC) residues). AuroraA auto-phosphorylation is initiated by several co-factors acting at different steps of mitosis. AroraB and AruroaC auto-phosphorylation are mediated by survivin and Borealin proteins.

Product Details
More Information
Applications Flow Cytometry
Clone AuroraABC-CC12
Format APC
Validated Reactivity Human
Cross Reactivity Predicted to work with mouse, rat and other homologues.
Detection Anti-Rabbit IgG
Clonality Monoclonal
Immunogen A synthetic phospho-peptide corresponding to residues surrounding human Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198)
Formulation 1X PBS, 0.09% NaN3, 0.2% BSA
Isotype Rabbit IgGk
Preparation Protein A+G
Recommended Usage For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells or 5 µL per 100 µL of staining volume. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.
Storage 2-8ºC
Pseudonyms Aurora kinase A/B/C, AURKA, AURKB, AURKC
Uniprot ID O14965
Q96GD4
Q9UQB9
References Hochegger H, et al., (2013) Open Biol. 3:120185.
Carmena M, et al., (2009) Curr Opin Cell Biol 21:796–805. 
Bolanos-Garcia VM. (2005) Int J Biochem Cell Biol. 37:1572–1577.
Kimmins S, et al., (2007) Mol Endocrinol. 2007;21(3):726–739.
Vader G, and Lens SMA. (2008) Biochim Biophys Acta. 1786:60–72.
Images
Cell Culture Products
Flow cytometric analysis of HeLa cells untreated and unstained as negative control (blue) or untreated red) or treated with nocodazole (green) and stained using Phospho-Aurora A (Thr288)/Aurora B (Thr232)/Aurora C (Thr198) antibody AuroraABC-CC12 APC conjugate. Cat. #2389.