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Phospho-Zap70/Syk (Tyr493/526) (H11) rabbit mAb
20 μL is enough antibody for at least 20 Western blots.
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|Description||ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1. Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of phospho Zap70.|
|Applications||Flow Cytometry, WB|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues.|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70|
|Validated Reactivity||Human, Mouse|
|Recommended Usage||1- 0.1 µg/ml. Optimum concentration should be determined by the user.|
|References||1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.
9. Law, C. L. et al. (1996) Mol. Cell. Biol. 16, 1305-1315.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11. Cat. #1161.
Western blot analysis of Jurkat cell extract untreated or treated with pervanadate using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11. Cat. #1161.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11 at 0.1 µg/mL. Cat #1161.
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