Phospho-Zap70 (Tyr493)/Syk (Tyr526) (H11) rabbit mAb
Catalog #: 1161
20 μL is enough antibody for at least 20 Western blots.
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ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.
|Applications||Flow Cytometry, WB|
|Validated Reactivity||Human, Mouse|
|Cross Reactivity||Predicted to work with mouse, rat, and other homologues.|
|Immunogen||A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70|
|Formulation||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA|
|Recommended Usage||1µg/mL – 0.001µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. See product image legends for additional information.|
|Pseudonyms||Tyrosine-protein kinase ZAP-70 , 70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, SRK, Tyrosine-protein kinase SYK, Spleen tyrosine kinase, p72-Syk|
|Uniprot ID||P43403 P43405|
|References||1. Brdicka T et al., (2005) Mol Cell Biol 25:4924–4933.
2. Chan AC et al., (1992) Cell 71:649–662.
3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797–9801.
4. Deindl S et al., (2007) Cell 129:735–746.
5. Elder ME et al., (1994) Science 264:1596–1599.
6. Negishi I et al., (1995) Nature 376:435–438.
7. Yokosuka T et al., (2005) Nat Immunol 6:1253–1262.
Western blot analysis of Jurkat cell extract untreated or treated with pervanadate using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11. Cat. #1161.
Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control Cat. #2141 (orange) or treated with imatinib (red) or with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11 at 0.1 µg/mL. Cat #1161.
Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11. Cat. #1161.
Phospho-Zap70 (Tyr319)/Syk (Tyr352) (A3) rabbit mAbCatalog #: 2076