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Purified anti-phospho-Zap70/Syk (Tyr493/Tyr526) rabbit mAb

phospho-Zap70/Syk (Tyr493/Tyr526)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using Phospho-Zap70 (Tyr493)/Syk (Tyr526) antibody Zap70Y493-H11. Cat. #1161.

  • phospho-Zap70/Syk (Tyr493/Tyr526)
Catalog Number: 1161
Clonality: Monoclonal
Applications: WB, Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 1161
Clone: Zap70Y493-H11
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr493 of human phospho Zap70
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: WB, Flow Cytometry
Recommended Usage: 1- 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: anti-rabbit IgG


ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1. Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of phospho Zap70.



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5.  Williams, B.L. et al. (1999) EMBO J 18, 1832-44.

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This product was added to our catalog on Sunday 31 July, 2016.

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20 ul (0.5mg/ml, more than 20 western blots) (1161S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (1161) $475.00 

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