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Purified anti-phospho-Zap70/Syk (Tyr319/Tyr352) (A3) rabbit mAb

phospho-Zap70/Syk (Tyr319/Tyr352) (A3)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or untreated (gray) or treated with H2O2 (orange) using 0.01µg/mL isotype control Cat. #2141, or untreated (red) or treated (green) using Phospho-Zap70 (Tyr319)/Syk (Tyr352) antibody, ZapY319-A3 (0.01 µg/mL), Cat. #2076.

  • phospho-Zap70/Syk (Tyr319/Tyr352) (A3)
Catalog Number: 2076
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
 
Catalog Number: 2076
Clone: Zap70Y319-A3
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr319/Tyr352 of human phospho Zap70/Syk.
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.01 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG

Description:

ZAP70 (Tyrosine-protein kinase ZAP-70, phospho Zap70) is a protein tyrosine kinase (PTK) that associates with the z subunit of the T cell antigen receptor (TCR) and undergoes tyrosine phosphorylation following TCR stimulation. Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck. ZAP70 contains two SH2-like domains with the PTK domain located at the C-terminus. It appears that both phospho Zap70 and Syk are recruited to the phosphorylated CD3 and z subunits after TCR stimulation. Phosphorylation of Tyr319 is required for the assembly of a phospho Zap70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association with PLC-gamma1.

Alternative name(s):
70 kDa zeta-chain associated protein
Syk-related tyrosine kinase
 

References:

  • 1. Brdicka T et al., (2005) Mol Cell Biol 25:4924–4933.

  • 2. Chan AC et al., (1992) Cell 71:649–662.

  • 3. Cheng AM et al., (1997) Proc Natl Acad Sci94:9797–9801.

  • 4. Deindl S et al., (2007) Cell 129:735–746.

  • 5. Elder ME et al., (1994) Science 264:1596–1599.

  • 6. Negishi I et al., (1995) Nature 376:435–438.

  • 7. Yokosuka T et al., (2005) Nat Immunol 6:1253–1262.

This product was added to our catalog on Monday 26 September, 2016.

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20 ul (0.5mg/ml) (2076S) $175.00 
200 ul (0.5mg/ml) (2076) $475.00 
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