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Purified anti-phospho-Stat3 (Tyr705) rabbit mAb

phospho-Stat3 (Tyr705)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue) or untreated (red) or treated with IFNα, IL-4 and pervanadate (green) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL). Cat. #1121.

  • phospho-Stat3 (Tyr705)
Catalog Number: 1121
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
 
Catalog Number: 1121
Clone: Stat3Y705-B12
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr705 of human phospho Stat3
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG

Description:

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development. Stat3 is constitutively activated in several human tumors and may be involved in oncogenic and anti-apoptotic functions. Phospho Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Phospho Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or maturation stage of the cells. Notably, Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.

References:

1. Heim, M.H. (1999) J. Recept. Signal Transduct. Res. 19:75-120.

2. Takeda, K. et al. (1997) Proc. Natl. Acad. Sci. USA. 94:3801-3804.

3. Catlett-Falcone, R. et al. (1999) Immunity. 10:105-115.

4. Garcia, R. and Jove, R. (1998) J. Biomed. Sci. 5:79-85.

5. Bromberg, J.F. et al. (1999) Cell. 98:295-303.

6. Darnell Jr., J.E. et al. (1994) Science. 264:1415-1421.

7. Ihle, J.N. (1995) Nature. 377:591-594.

8. Wen, Z. et al. (1995) Cell. 82:241-250.

9. Yokogami, K. et al. (2000) Curr. Biol. 10:47-50.

10. Biethahn, S. et al. (1999) Exp. Hematol. 27:885-894.

This product was added to our catalog on Sunday 31 July, 2016.

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20 ul (0.5mg/ml) (1121S) $175.00 
200 ul (0.5mg/ml) (1121) $475.00 
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