||A synthetic phospho-peptide corresponding to residues surrounding Ser807/Ser811 of human phospho Rb
||Predicted to work with mouse, rat and other homologues.
||1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
||WB, Flow Cytometry
||1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Retinoblastoma protein (Rb, phospho Rb) is a tumor suppressor protein that is inactivated in a numbr of diverse cancers. The antiproliferative activity of Rb is mediated by its ability to inhibit the transcription of genes that are required for cell cycle progression. Rb contains conserved sites that are phosphorylated by cyclin-dependent kinases (CDKs). CDK phosphorylation typically promotes protein-protien interations through creation of a phospho-epitope that becomes structured upon binding its target. However Rb phosphorylation distrupts interactions with its binding partners. When it is phosphorylated, phospho Rb is inactivated and allows execcisve cell growth that is seen in cancer cells. Sixteen potential sites for CDK-mediated phosphorylation exist in Rb and twelve of these sites have been shown to be phosphorylated in vivo.
1. Burkhart DL et al., (2008) Nat Rev Cancer. 8:671–682.
2. Knudsen ES (2008) Nat Rev Cancer. 8:714–724.
3. Mittnacht S. (1998) Curr Opin Genet Dev. 8:21–27.
4. Weinberg RA. (1995) 81:323–330.
5. Henley SA et al., (2012) Cell Div., 7:10.
This product was added to our catalog on Friday 30 September, 2016.