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Conjugated anti-phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) FITC rabbit mAb

phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) FITC

Flow cytometric analysis of Ramos cells, untreated (red) or treated with pervanadate (green) using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) FITC conjugated antibody PI3KY458-1A11. Cat. #2068.

Catalog Number: 2068
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Conjugated
Conjugate: FITC
 
Catalog Number: 2068
Clone: PI3KY458-1A11
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.09% NaN3, 0.2% BSA
Storage: 2-8ºC
Applications: Flow Cytometry
Recommended Usage: For flow cytometric staining, the suggested use of this reagent is 5 μl per million cells or 5 μl per 100 μl of staining vlolume. It is recommended that the reagent be titrated for optimal performance for each application
Detection: Anti-Rabbit IgG

Description:

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

References:

1.  Cantley, L.C. (2002) Science 296, 1655-7.

2.  Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.

3.  Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.

This product was added to our catalog on Friday 30 December, 2016.

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10 tests (2068S) $99.00 
100 tests (2068) $299.00 
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