||A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85
||Predicted to work with mouse, rat, and other homologues.
||1X PBS, 0.09% NaN3, 0.2% BSA
||For flow cytometric staining, the suggested use of this reagent is 5 μl per million cells or 5 μl per 100 μl of staining vlolume. It is recommended that the reagent be titrated for optimal performance for each application
Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.
1. Cantley, L.C. (2002) Science 296, 1655-7.
2. Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
3. Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
This product was added to our catalog on Friday 30 December, 2016.