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Purified anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) rabbit mAb

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue) or treated with U0126 (red) or treated with PMA (green) using Phospho-ERK1/2 (Thr202/Tyr204) antibody ERK12T202Y204-A11. Cat. #1111.

  • Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
  • Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)
Catalog Number: 1111
Clonality: Monoclonal
Applications: WB, Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 1111
Clone: ERK12T202Y204-A11
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr202/Tyr204 of human phospho Erk1/2.
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: WB, Flow Cytometry
Recommended Usage: 1- 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: anti-rabbit IgG


MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. The p44/42 MAPK (Erk1/2) are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. Phospho ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upstream MEK1 and MEK2 activate p44 and p42 (phospho ERK1/2) through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively.  p44/42 (ERK1/2)  are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs, along with MEK inhibitors, such as U0126 and PD98059.




1. Warnecke N, et al. (2012) EMBO Rep. 13: 386-91.

2. Mebratu Y, et al. (2009) Cell Cycle 8:1168-75.

3. Ebisuya M, et al. (2005) J Cell Sci. 118: 2997-3002.


This product was added to our catalog on Tuesday 19 July, 2016.

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Size(s) Price
20 ul (0.5mg/ml, more than 20 western blots) (1111S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (1111) $475.00 

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