Contact Us

Purified anti-phospho-MKK7 (Ser271/Thr275) rabbit mAb

phospho-MKK7 (Ser271/Thr275)

Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with EGF (green) using Phospho-MKK7(S271/T275) antibody MKK7S271T275-R4F9 at 0.5 µg/mL. Cat. #2156.

  • phospho-MKK7 (Ser271/Thr275)
Catalog Number: 2156
Clonality: Monoclonal
Applications: WB, Flow Cytometry
Reactivity: Rat, Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 2156
Clone: MKK7S271T275-R4F9
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser271/Thr275 of human phospho MKK7
Reactivity: Rat,Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: WB, Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


MKK7 is a dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. It is an essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that phospho MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Phospho MKK7 has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.



1. Wu Z, et. al. (1997) Mol. Cell. Biol. 17:7407-7416.

2. Lu X,  et al. (1997) J. Biol. Chem. 272:24751-24754.

3. Foltz I.N., et al. (1998) J. Biol. Chem. 273:9344-9351.



This product was added to our catalog on Tuesday 14 February, 2017.

Most Viewed Product

Product Price Options

Size(s) Price
20 ul (0.5mg/ml, more than 20 western blots) (2156S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (2156) $475.00 

Shipping Information

$49 FedEx Standard Overnight flat rate for as many products shipped within the United States.

$25 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.