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Purified anti-phospho-MET (Tyr 1234/Tyr1235) rabbit mAb

phospho-MET (Tyr 1234/Tyr1235)

Flow cytometric analysis of Ramos cells, secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using 0.005 ug/mL Phospho-MET(Y1234/1235) antibody METY12341235-6F11. Cat. # 2216.

  • phospho-MET (Tyr 1234/Tyr1235)
Catalog Number: 2216
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
 
Catalog Number: 2216
Clone: MetY12341235-6F11
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr1234/Tyr1235 of human phospho Met
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein AG
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbt IgG

Description:

The Mesenchymal Epithelial Transition (MET) receptor belongs to a family of receptor tyrosine kinases (RTKs). Dyregulation of the MET signaling pathway occurs in wide range of human tumors (1,2).  The MET receptor is a glycoprotein heterodimer consisting of an extracellular α-subunit linked to transmembrane β-subunit by a disulfide bond (3).  The MET receptor is widely expressed on the surface of epithelial and endothelial cells.  MET ligand is known as the hepatocyte growth factor (HGF), also called the scatter factor (SF).  HGF/SF is a serine protease and produced in cells of mesenchymal origin. HGF is secreted as a s ingle chain, biologically inactive protein which is further processed into its mature form by a cleavage process catalyzed by extracellular proteases (4-6).  MET and its ligand HGF are involved in several normal and pathological processes, such as fetal liver, placenta and muscle development, as well as development of the nervous system (7-9).  MET signal transduction is involved in tumor growth, invasion, resistance to therapy, angiogenesis and specially in the generation and maintenance of cancer stem cells (CSCs) (10-12).

Upon HGF binding, the MET receptor at the plasma membrane forms a stable dimer of two molecules of MET which leads to subsequent activation and trans-phosphorylation of the two tyrosine residues in the catalytical regions Y1234 and Y1235, followed by trans-phosphorylation of two docking tyrosines (Y1349 and Y1356) in the carboxy-terminal site. These phosphorylations enable MET to bind to multiple substrates and activate a variety of signaling pathways including MAPK, PI3K-AKT cascades, STAT and NF-κB (13-15).

 

References:

1. Peters S, and Adjei AA. (2012) Nat. Rev. Clin. Oncol. 9:314–26.

2. Maroun CR, and Rowlands T. (2014) Pharmacol. Ther. 142:316–38. 

3. Gherardi E, et al., (2012) Nat. Rev. Cancer. 12:89–103.

4. Gelsomino F, et al., (2014) Cancers (Basel) 6:2100–15.

5. Trusolino L, et al., (2010) Nat. Rev. Mol. Cell Biol. 11:834–48.

6. Furlan A, et al., (2014) Cancer Res. 74:6737–44. 9. 

7. Parikh RA, et al., (2014) Onco. Targets Ther. 7:969–83.

8. Uehara Y, et al., (1995) Nature 373:702–5.

9. Schmidt C, et al., (1995) Nature 373:699–702.

10. Zhang Y, et al., (2105) Agents for Cancer Treatment Biomedicines. 3:149–181.

11. Bussolino F, et al., (1992) J. Cell Biol. 119:629–41.

12. Boccaccio C, and Comoglio PM. (2014) Curr. Opin. Cell Biol. 31:98–105.

13. Ponzetto C, et al., (1994) Cell 77:261–71.

14. Johnson GL, and Lapadat R. (2002) Science. 298:1911–2. 

15. Muller M, ett al., (2002) Mol. Cell Biol. 22:1060–72.

 

This product was added to our catalog on Sunday 04 December, 2016.

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20 ul (0.5mg/ml, more than 20 western blots) (2216S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (2216) $475.00 
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