Contact Us

Purified anti-phospho-CrkL (Tyr207) rabbit mAb

phospho-CrkL (Tyr207)

Flow cytometric analysis of K562 cells, secondary antibody only negative control (blue) or imatinib (red) or treated with pervanadate (green) using Phospho-CrkL (Tyr207) antibody CrkLY207-G4 at 0.05 µg/mL. Cat. #2091.

  • phospho-CrkL (Tyr207)
  • phospho-CrkL (Tyr207)
  • phospho-CrkL (Tyr207)
Catalog Number: 2091
Clonality: Monoclonal
Applications: WB, Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 2091
Clone: CrkLY207-G4
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr207 of human phospho CrkL
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: WB, Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


CrkL  (CRK Like Proto-Oncogene, Adaptor Protein), which is a V-crk avian sarcoma virus CT10 oncogene homolog-like adapted protein of CRK family, belongs to a novel class of regulatory proteins which include v-Crk and its cellular homologs Crk I and Crk II. Protein structure of CrkL contains several SH2 and SH3 domains. Phospho CrkL plays a central role in cell proliferation, cell adhesion, migration and phagocytic and endocytic pathways (1). Dysfunction of CRKL plays key roles in a variety of human diseases including human malignancies, e.g. chronic myelogenous leukemia, colon cancer and prostate cancer (2-5). Phospho CrkL is known to regulate signaling through interactions of its SH3 domain with proline-rich motif containing proteins, such as SOS, C3G, and p85. In CrkL-induced cell transformation, CrkL association with these proteins and activates SOS1-RAS-RAF-ERK and Src-C3G-RAP1 signaling pathways. The SH2 and SH3 domains associate with effector proteins containing phosphorylated tyrosine (pTyr)-Xaa-Xaa-Pro and Pro-Xaa-Xaa-Pro motifs respectively. The SH3 domains are separated by a linker containing Tyr207 which is phosphorylated by Abl tyrosine kinase. 



1. Wang J, et al. (2013)  Chemico-biological interactions. 206: 230–8.

2. Ungewiss C, et al. (2016) Scientific reports. 6:18652. 

3. Birge RB, et al. (2009) Cell communication and Signaling: CCS. 7:13. 3 

4. Panigrahi S, et al. (2012)  PLOS ONE. 7:e28395,

5. Singer CF, et al. (2006) Oncol. Rep. 15: 353-9. 


This product was added to our catalog on Tuesday 25 October, 2016.

Most Viewed Product

Product Price Options

Size(s) Price
20 ul (0.5mg/ml, more than 20 western blots) (2091S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (2091) $475.00 

Shipping Information

$49 FedEx Standard Overnight flat rate for as many products shipped within the United States.

$25 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.