Contact Us

Purified anti-phospho-Chk1 (Ser317) (F10) rabbit mAb

phospho-Chk1 (Ser317) (F10)

Flow cytometric analysis of Hela cells, secondary antibody only negative control (blue) or treated imatinib (red) or treated with pervanadate (green) using Phospho-Chk1 (S317) antibody Chk1S317-F10, 0.1 µg/mL. Cat. #2151.

  • phospho-Chk1 (Ser317) (F10)
Catalog Number: 2151
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 2151
Clone: Chk1S317-F10
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser317 of human phospho Chk1
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


The act of DNA damaged response and cell cycle checkpoints requires the activation of four protein kinases that form the canonical ATR-Chk1 and ATM-Chk2 pathways. ATR activation requires the generation of structures containing single strand DNA (ssDNA) adjacent to double strand DNA (dsDNA). Such ssDNA is coated with replication protein A complex and attracts ATR (1,2). The accumulation of ATR to damage sites results in initial activation of ATR. ATR phosphorylates proteins at the ssDNA which are called checkpoint regulators. The accumulation and phosphorylation of these checkpoint regulators further stimulates the catalytic activity of ATR. ATR-induced Chk1 phosphorylation likely occurs at the sites of DNA damage on chromatin (3-5)The activated ATR phosphorylates Chk1 at Ser317 and Ser345 in its C-terminal regulatory domain. Phospho Chk1 is critical for DNA damage checkpoint activation, replication control, and cell viability (6-8). Functionally, ATR-mediated phosphorylation elevates phospho Chk1 catalytic activity. The N-terminal catalytic domain of Chk1 adopts an open kinase conformation and the deletion of C-terminal domain increases Chk1 catalytic activity.



1. Caprelli ML, et al. (2013) Cell Cycle, 12: 916-22.

2. Capasso H, et al. (2002) J. Cell Sci. 115: 4555-64.

3. Carrassa L, et al. (2011) Cell Cycle 10: 2121-8.

4. Chen MS, et al. (2003) Mol. Cell Biol. 23: 7488-97.

5. Ciccia A, et al. Mol. Cell 40: 179-204.

6. Cimprich CA, (2014) Oncogene 33: 3351-60

7. Cremona CA, et al. (2014) Oncogene 33: 3351-60.

8. Niida H, et al. (2007) Mol. Cell Biol. 27: 2572-81.




This product was added to our catalog on Tuesday 31 January, 2017.

Most Viewed Product

Product Price Options

Size(s) Price
20 ul (0.5mg/ml) (2151S) $175.00 
200 ul (0.5mg/ml) (2151) $475.00 

Shipping Information

$49 FedEx Standard Overnight flat rate for as many products shipped within the United States.

$25 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.