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Purified anti-phospho-Btk (Tyr223) rabbit mAb

phospho-Btk (Tyr223)

Flow cytometric analysis of U937 cells, secondary antibody only negative control (blue) or untreated (red) or treated with IFNa, IL4 and PV (green) using 5 ng/mL of Phospho-Btk (Tyr223) antibody BtkY223-B4 Cat. #1171.

  • phospho-Btk (Tyr223)
Catalog Number: 1171
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 1171
Clone: BtkY223-B4
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human phospho Btk
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


Bruton's tyrosine kinase (Btk) plays a major role in B cell development. B cell activation by various ligands is accompanied by Btk membrane translocation, where ransient phosphorylation at two sites, Tyr551 and Tyr223 create phospho Btk. Tyr551, in the activation loop, is transphosphorylated by the Src family of tyrosine kinases, leading to autophosphorylation at Tyr223. Tyr223 phosphorylation facilitates phospho Btk interaction with activated Syk. Defects in Btk are the cause of X-linked agammaglobulinemia (XLA); also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). 



1.  Khan WN, (2001) Immunol. Res. 23: 147-56.

2.  Lewis CM, et al. (2001) Curr. Opin. Immunol. 13: 317-25.

3.  Salim K, et al. (1996) EMBO J. 15: 6241-50.

4.  Rameh LE, et al. (1997) J. Biol. Chem. 272: 22059-66.

5.  Várnai P, et al. (1999) J. Biol. Chem. 274: 10983-9.

6.  Rawlings, D.J. et al. (1996) Science 271: 822-5.

7.  Park H, et al. (1996) Immunity 4: 515-25.


This product was added to our catalog on Tuesday 31 January, 2017.

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20 ul (0.5mg/ml) (1171S) $175.00 
200 ul (0.5mg/ml) (1171) $475.00 

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