Contact Us

Purified anti-phospho-Akt1 (Ser473) (C7) rabbit mAb

phospho-Akt1 (Ser473) (C7)

Flow cytometric analysis of U937 cells, secondary antibody only negative control (blue) or treated with LY294002 (red) or with calyculin A (green) using Phospho-Akt (Ser473) antibody AktS473-C7. Cat. #1131.

Catalog Number: 1131
Clonality: Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 1131
Clone: AktS473-C7
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser473 of human phospho Akt1
Reactivity: Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


Akt also known as PKB (Protein kinase B) or RAC-PK (Related to the A and C kinases) is a serine/threonine kinases that contains a pleckstrin homology (PH) domain. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. Phospho-Akt promotes cell survival by inhibiting apoptosis. Specifically, phospho-Akt1 has been shown to phosphorylate Bad, a member of the Bcl-2 family that promotes cell death. This phosphorylation results in the inactivation of the proapoptotic function of Bad. The Akt/phospho Akt molecule is thus considered to link extracellular survival signals (growth factors) with the apoptotic machinery (BAD). Akt is also a key mediator of the metabolic effects of insulin. Additionally, Akt has been referred to as an oncogene because it has increased activity in a number of tumors. This antibody recognizes phospho Akt phosphorylated at Ser473. This phosphorylation site is shared by all three isoforms of phospho Akt. The homologous phosphorylation sites in Akt2 and Akt3 are S474 and S472, respectively.  



1. Franke TF, et al. (1997) Cell 88: 435-7.

2. Burgering, B.M. and Coffer, P.J. (1995) Nature 376: 599-602.

3.Franke TF, et al. (1995) Cell 81: 727-36.

4. Alessi DR, et al. (1996) EMBO J 15: 6541-51.

5. Sarbassov DD, et al. (2005) Science 307: 1098-101.

6. Jacinto E, et al. (2006) Cell 127: 125-37.

7. Cardone MH, et al. (1998) Science 282: 1318-21.

8. Brunet A, et al. (1999) Cell 96: 857-68.

9. Zimmermann S and Moelling K, (1999) Science 286: 1741-4.

10. Cantley LC and Neel BG (1999) Proc Natl Acad Sci USA 96: 4240-5.

11. Vlahos CJ, et al. (1994) J Biol Chem 269: 5241-8.

12. Hajduch E, et al. (2001) FEBS Lett 492: 199-203.

13. Cross DA, et al. (1995) Nature 378: 785-9.

14. Diehl JA, et al. (1998) Genes Dev 12: 3499-511.

15. Gesbert F, et al. (2000) J Biol Chem 275: 39223-30.

16. Zhou BP, et al. (2001) Nat Cell Biol 3: 245-52.

17. Navé BT, et al. (1999) Biochem J 344: 427-31.

18. Inoki K, et al. (2002) Nat Cell Biol 4: 648-57.

19. Manning BD, et al. (2002) Mol Cell 10: 151-62.


This product was added to our catalog on Sunday 31 July, 2016.

Most Viewed Product

Product Price Options

Size(s) Price
20 ul (0.5mg/ml) (1131S) $175.00 
200 ul (0.5mg/ml) (1131) $475.00 

Shipping Information

$49 FedEx Standard Overnight flat rate for as many products shipped within the United States.

$25 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.