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Purified anti-phospho-4E-BP1 (Thr37/Thr46) rabbit mAb

phospho-4E-BP1 (Thr37/Thr46)

Flow cytometric analysis of Jurkat cells, secondary antibody only negative control (blue), or treated with LY294002, Wortmannin and U0126 (red) or with FBS (green) using Phospho-4E-BP1 (Thr37/Thr46) antibody 4EB1T37T46-A5 at 0.1µg/mL. Cat. #2041.

  • phospho-4E-BP1 (Thr37/Thr46)
  • phospho-4E-BP1 (Thr37/Thr46)
  • phospho-4E-BP1 (Thr37/Thr46)
  • phospho-4E-BP1 (Thr37/Thr46)
Catalog Number: 2041
Clonality: Monoclonal
Applications: IHC, WB, Flow Cytometry
Reactivity: Rat, Mouse, Human
Format: Purified
Conjugate: Unconjugated
Catalog Number: 2041
Clone: 4EB1T37T46-A5
Isotype: Rabbit IgG1κ
Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Thr37/46 of human phospho 4E-BP1.
Reactivity: Rat,Mouse,Human
Cross Reactivity: Predicted to work with mouse, rat, and other homologues.
Preparation: Protein A+G
Formulation: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Storage: -20ºC
Applications: IHC, WB, Flow Cytometry
Recommended Usage: 1.0 - 0.1 µg/ml. Optimum concentration should be determined by the user.
Detection: Anti-Rabbit IgG


Eukaryotic translation initiation factor 4E-binding protein 1 (PHAS-1, EIF4EBP1, eIF4E-binding protein 1, phospho 4E-BP1) is one member of a family of translation repressor proteins encoded by the EIF4EBP gene (1). The protein directly inhibits cap-dependent translation by binding eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multi-subunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of cap-dependent mRNA translation(2). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of phospho 4E-BP1 to eIF4E, it is thought to prime phospho 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.




1. Pause A, et al. (1994) Nature 371: 762-7
2. Mader S, et al. (1995) Mol. Cell. Biol. 15: 4990–7.


This product was added to our catalog on Sunday 31 July, 2016.

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20 ul (0.5mg/ml, more than 20 western blots) (2041S) $175.00 
200 ul (0.5mg/ml, more than 200 western blots) (2041) $475.00 

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