Monoclonal Antibody Engineering
Our scientists will work closely with you during all phases of the project to ensure success. We want to be an integral part of your R&D team. We value open communication at all project stages and will always freely discuss our approaches to possible challenges. We love the work that we do, and we want our clients to feel the same level of excitement.
The Abwiz Bio monoclonal antibody engineering team is comprised of multiple Ph.D. scientists, collectively possessing decades of experience developing unique antibodies using phage display technology. We enjoy tackling interesting, new challenges and solving difficult problems.
Recombinant monoclonal antibody engineering
We offer monoclonal antibody engineering and production services including:
Creation of a recombinant antibody from an existing hybridoma
Creation of a recombinant antibody from an antibody fragment (scFv, Fab)
Rapid (4 weeks) recombinant antibody manufacturing services
Based on a proprietary transient CHO expression system which utilizes our proprietary serum-free, defined cell culture media
Recombinant antibody modifications:
Affinity maturation including human, mouse and rabbit antibodies
Chimeric antibody production
Specificity engineering (e.g. discrimination between human and mouse antigens)
Fc receptor binding modification
Immunoglobulin isotype/class or subclass switching
Optimization of expression
Abwiz Bio's CHO expression system is compatible with any species of recombinant antibody. We routinely achieve high yields and high purity for a variety of human, mouse and rabbit antibodies.
For monoclonal antibody engineering projects that require amino acid mutagenesis (e.g. affinity maturation or expression optimization), Abwiz Bio's highly optimized Fab expression vector is ideal for library creation. Screening can be performed after phage-based selection from large, unbiased libraries (109-1011 in size), or Fabs can be screened from small, focused libraries. The ability to screen for Fab activity prior to the IgG production step can save enormous time and resources during lead candidate identification.